Plasma membrane translocation of a protein needle based on a triple-stranded β-helix motif.
Plasma membrane translocation of a protein needle based on a triple-stranded β-helix motif.
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基于三链β-螺旋基序的蛋白质针的质膜易位。
DOI：
10.1039/c4mb00293h
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Kitagawa S and Ueno T
中科院分区：
文献类型：
--
作者：
Sanghamitra. NJM;Inaba H;Arisaka F;Wang DO;Kanamaru S;Kitagawa S and Ueno T
由于细胞膜的屏障，质膜移位具有挑战性。与合成的细胞穿透材料相反，加尾噬菌体使用细胞穿刺蛋白质针穿刺细胞膜作为DNA注射过程的初始步骤。细胞穿刺蛋白质针被认为在天然细胞中保持功能。在本文中，我们发现一个16 nm长的噬菌体T4衍生的蛋白质针自发易位通过活细胞膜。β-螺旋蛋白针（β-PN）内化到缺乏内吞机制的人红细胞中。通过比较β-PN的细胞摄取与修饰的表面电荷，表明当其具有对应于-16 mV的zeta电位值的负电荷时，摄取效率最大。在HeLa细胞中，β-PN的摄取结合了内吞作用独立机制和部分巨胞饮依赖性。当β-PNs的表面电荷被修饰为正或负时，摄取的内吞依赖性增加。因此，这些结果表明，天然DNA注入机制可以作为设计具有定制机制的新型细胞穿透材料的灵感。
Plasma membrane translocation is challenging due to the barrier of the cell membrane. Contrary to the synthetic cell-penetrating materials, tailed bacteriophages use cell-puncturing protein needles to puncture the cell membranes as an initial step of the DNA injection process. Cell-puncturing protein needles are thought to remain functional in the native phages. In this paper, we found that a bacteriophage T4 derived protein needle of 16 nm length spontaneously translocates through the living cell membrane. The β-helical protein needle (β-PN) internalizes into human red blood cells that lack endocytic machinery. By comparing the cellular uptake of β-PNs with modified surface charge, it is shown that the uptake efficiency is maximum when it has a negative charge corresponding to a zeta potential value of −16 mV. In HeLa cells, uptake of β-PN incorporates endocytosis independent mechanisms with partial macropinocytosis dependence. The endocytosis dependence of the uptake increases when the surface charges of β-PNs are modified to positive or negative. Thus, these results suggest that natural DNA injecting machinery can serve as an inspiration to design new class of cell-penetrating materials with a tailored mechanism.
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影响因子：
3.7
作者：
Morris MJ;Liu D;Weaver LM;Board PG;Casarotto MG
通讯作者：
Casarotto MG
影响因子：
4.8
作者：
Futaki, S;Suzuki, T;Sugiura, Y
通讯作者：
Sugiura, Y
噬菌体 P22 DNA 跨细胞质膜的运输
影响因子：
3.2
作者：
G. L. Perez;Bao;M. Slater;S. Maloy
通讯作者：
S. Maloy
影响因子：
4
作者：
Fuchs, Stephen M.;Raines, Ronald T.
通讯作者：
Raines, Ronald T.
DOI：
--
发表时间：
2006
期刊：
--
影响因子：
--
作者：
Iroon Polytechniou-
通讯作者：
Iroon Polytechniou-