Mechanisms and consequences of pH-mediated cell regulation.
Mechanisms and consequences of pH-mediated cell regulation.
复制标题
pH 介导的细胞调节的机制和后果。
作者：
W. Busa
氢离子的活性（通常以pH值表示）一直被认为是水化学中一个重要的基本参数。虽然从逻辑上讲，同样的情况也适用于被称为细胞生物学的水化学的一个相当专门的分支，但在完整细胞的范围内测量这一参数的技术困难，极大地阻碍了对细胞内pH值（pHi）在调节细胞行为方面可能发挥的作用的实验评估。幸运的是，最近的技术进步为细胞生物学家提供了越来越多的测量pHi的技术（参见参考文献46和57的评论），并且它们的应用已经发现了大量（并且仍在增长）的实例，其中pHi随细胞生命中定义的事件而变化。在总共19个属中，这些事件包括配子激活和受精、包囊和孢子萌发、各种刺激-反应耦合的例子、细胞周期的进展以及细胞能量平衡的变化（参见参考文献13和47）。最近，在粘菌Dictyostelium的分化（24,32）、线虫精细胞向活跃精子的成熟（78）、黏菌绒泡菌的趋化反应（28）、哺乳动物精子的运动起始(5)、藻菌Blastocladiella游动孢子的萌发（74）以及许多哺乳动物细胞系的有丝分裂刺激（见W. H. Moole - naar, 2014）等一系列事件中，pHi的变化被检测或推断出来。本卷)。确凿的证据表明，这些pHi变化在伴随的过程中起着调节作用，这在医学上是很难实现的
The activity of hydrogen ions (commonly expressed in terms of pH) has long been recognized as a parameter of fundamental importance in aqueous chemis try. Although the same would logically be expected to be true for that rather specialized branch of aqueous chemistry known as cell biology, the technical difficulties of measuring this parameter within the confines of the intact cell have greatly retarded experimental assessment of the roles intracellular pH (pHi) might play in regulating the behavior of cells. Fortunately, recent tech nical advances have provided cell biologists with a growing armory of tech niques with which to measure pHi (see References 46 and 57 for reviews), and their application has uncovered a large (and still growing) number of instances in which pHi changes accompany defined events in the lives of cells. In a total of nineteen genera these events include gamete activation and fertilization, cyst and spore germination, various examples of stimulus-response coupling, pro gression through the cell cycle, and changes in cellular energy balance (see References 13 and 47 for review). Most recently, pHi changes have been detected or inferred in the sequence of events involved in differentiation of the slime mold Dictyostelium (24,32), in the maturation of nematode spermatids to active spermatozoa (78), in the chemotactic response of the myxomycete Physarum (28), in motility initiation of mammalian spermatozoa (5), in germination of zoospores of the phycomycete Blastocladiella (74), and in mitogenic stimulation of numerous mammalian cell lines (see W. H. Moole naar, this volume). Conclusive evidence that these pHi changes play a regula tory role in the processes they accompany has proven difficult to achieve, in
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影响因子：
2.7
作者：
S. Ward;E. Hogan;G. A. Nelson
通讯作者：
S. Ward;E. Hogan;G. A. Nelson
血清、缓激肽和加压素刺激人成纤维细胞释放磷酸肌醇。
DOI：
10.1016/0006-291x(84)90280-8
发表时间：
1984
影响因子：
3.1
作者：
Vicentini,LM;Villereal,ML
通讯作者：
Villereal,ML
白三烯 B4 动员钙，但不会分解多磷酸肌醇，也不在兔中性粒细胞中产生磷脂酸。
DOI：
10.1073/pnas.81.19.5966
发表时间：
1984
影响因子：
11.1
作者：
Volpi,M;Yassin,R;Tao,W;Molski,TF;Naccache,PH;Sha'afi,RI
通讯作者：
Sha'afi,RI
间隙连接结构和细胞间耦合调节：是否涉及钙调蛋白？
DOI：
--
发表时间：
1984
期刊：
Federation proceedings
影响因子：
--
作者：
Peracchia,C;Bernardini,G
通讯作者：
Bernardini,G