The phosphorylated regulator of chemotaxis is crucial throughout biofilm biogenesis in Shewanella oneidensis.
The phosphorylated regulator of chemotaxis is crucial throughout biofilm biogenesis in Shewanella oneidensis.
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DOI：
10.1038/s41522-020-00165-5
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发表时间：
2020-11-13
影响因子：
9.2
通讯作者：
Jourlin-Castelli C
中科院分区：
文献类型：
--
作者：
Boyeldieu A;Ali Chaouche A;Ba M;Honoré FA;Méjean V;Jourlin-Castelli C
锥状希瓦氏菌趋化系统的核心是由CheA3激酶和CheY3调节子组成。当适当时，CheA3使CheY3磷酸化，CheY3进而与鞭毛的转子结合以改变游泳方向。在本研究中，我们证明了磷酸化CheY3(CheY3-P)在固体表面相关生物膜(SSA-Biofilm)的生物发生过程中也发挥了重要作用。事实上，在ΔcheY3菌株中，这种生物膜的形成被取消。利用模拟磷的CheY3D56E突变体，我们证明了Chey-P在生物膜的整个生物发生过程中都是必需的，但在这一过程中，CheY3的磷酸化不依赖于CheA3。我们最近发现CheY3与两个二鸟苷环化酶(DGC)和c-di-GMP效应因子MxdA相互作用，可能通过MXD机制触发胞外多糖的合成。在这里，我们发现了另外两个参与SSA生物膜发育的DGC，并表明其中一个与CheY3相互作用。因此，我们建议CheY3-P与DGC共同作用来控制SSA生物膜的形成。有趣的是，两个同源的Chey调节器补充了ΔcheY3菌株的生物膜缺陷，支持了生物膜的形成可能涉及其他细菌中的Chey调节器的想法。
The core of the chemotaxis system of Shewanella oneidensis is made of the CheA3 kinase and the CheY3 regulator. When appropriated, CheA3 phosphorylates CheY3, which, in turn, binds to the rotor of the flagellum to modify the swimming direction. In this study, we showed that phosphorylated CheY3 (CheY3-P) also plays an essential role during biogenesis of the solid-surface-associated biofilm (SSA-biofilm). Indeed, in a ΔcheY3 strain, the formation of this biofilm is abolished. Using the phospho-mimetic CheY3D56E mutant, we showed that CheY-P is required throughout the biogenesis of the biofilm but CheY3 phosphorylation is independent of CheA3 during this process. We have recently found that CheY3 interacts with two diguanylate cyclases (DGCs) and with MxdA, the c-di-GMP effector, probably triggering exopolysaccharide synthesis by the Mxd machinery. Here, we discovered two additional DGCs involved in SSA-biofilm development and showed that one of them interacts with CheY3. We therefore propose that CheY3-P acts together with DGCs to control SSA-biofilm formation. Interestingly, two orthologous CheY regulators complement the biofilm defect of a ΔcheY3 strain, supporting the idea that biofilm formation could involve CheY regulators in other bacteria.
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影响因子：
10.5
作者：
Dahlstrom KM;O'Toole GA
通讯作者：
O'Toole GA
影响因子：
3.7
作者：
Baaziz H;Gambari C;Boyeldieu A;Ali Chaouche A;Alatou R;Méjean V;Jourlin-Castelli C;Fons M
通讯作者：
Fons M
影响因子：
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作者：
Goedeke, Julia;Paul, Kristina;Thormann, Kai M.
通讯作者：
Thormann, Kai M.
影响因子：
2.7
作者：
Allison, DG
通讯作者：
Allison, DG
影响因子：
9.8
作者：
De N;Pirruccello M;Krasteva PV;Bae N;Raghavan RV;Sondermann H
通讯作者：
Sondermann H