Protective CD8(+) T-cell response against Hantaan virus infection induced by immunization with designed linear multi-epitope peptides in HLA-A2.1/K(b) transgenic mice.
Protective CD8(+) T-cell response against Hantaan virus infection induced by immunization with designed linear multi-epitope peptides in HLA-A2.1/K(b) transgenic mice.
复制标题
HLA-A2.1/Kb 转基因小鼠中设计的线性多表位肽免疫诱导针对汉坦病毒感染的保护性 CD8 T 细胞反应
DOI：
10.1186/s12985-020-01421-y
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发表时间：
2020-10-07
期刊：
影响因子：
4.8
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Ma Y;Tang K;Zhang Y;Zhang C;Cheng L;Zhang F;Zhuang R;Jin B;Zhang Y
背景
Background
An effective vaccine that prevents disease caused by hantaviruses is a global public health priority, but up to now, no vaccine has been approved for worldwide use. Therefore, novel vaccines with high prophylaxis efficacy are urgently needed.
Methods
Herein, we designed and synthesized Hantaan virus (HTNV) linear multi-epitope peptide consisting of HLA-A*02-restricted HTNV cytotoxic T cell (CTL) epitope and pan HLA-DR-binding epitope (PADRE), and evaluated the immunogenicity, as well as effectiveness, of multi-epitope peptides in HLA-A2.1/Kb transgenic mice with interferon (IFN)-γ enzyme-linked immunospot assay, cytotoxic mediator detection, proliferation assay and HTNV-challenge test.
Results
The results showed that a much higher frequency of specific IFN-γ-secreting CTLs, high levels of granzyme B production, and a strong proliferation capacity of specific CTLs were observed in splenocytes of mice immunized with multi-epitope peptide than in those of a single CTL epitope. Moreover, pre-immunization of multi-epitope peptide could reduce the levels of HTNV RNA loads in the liver, spleen and kidneys of mice, indicating that specific CTL responses induced by multi-epitope peptide could reduce HTNV RNA loads in vivo.
Conclusions
This study may provide an important foundation for the development of novel peptide vaccines for HTNV prophylaxis.
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影响因子：
3.7
作者：
Kashi VP;Jacob RA;Shamanna RA;Menon M;Balasiddaiah A;Varghese RK;Bachu M;Ranga U
通讯作者：
Ranga U
DOI：
10.1084/jem.20031111
发表时间：
2004-06-07
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Arens R;Schepers K;Nolte MA;van Oosterwijk MF;van Lier RA;Schumacher TN;van Oers MH
通讯作者：
van Oers MH
影响因子：
5.4
作者：
Lindgren, Therese;Ahlm, Clas;Bjorkstrom, Niklas K.
通讯作者：
Bjorkstrom, Niklas K.
影响因子：
7.6
作者：
Jonsson, Colleen B.;Hooper, Jay;Mertz, Gregory
通讯作者：
Mertz, Gregory
影响因子：
4.7
作者：
Laenen, Lies;Vergote, Valentijn;Maes, Piet
通讯作者：
Maes, Piet