Molar loss and powder diet leads to memory deficit and modifies the mRNA expression of brain-derived neurotrophic factor in the hippocampus of adult mice.
Molar loss and powder diet leads to memory deficit and modifies the mRNA expression of brain-derived neurotrophic factor in the hippocampus of adult mice.
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DOI：
10.1186/s12868-016-0319-y
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发表时间：
2016-12-05
期刊：
影响因子：
2.4
通讯作者：
Tsuga K
中科院分区：
文献类型：
--
作者：
Takeda Y;Oue H;Okada S;Kawano A;Koretake K;Michikawa M;Akagawa Y;Tsuga K
众所周知，牙齿脱落是阿尔茨海默病的一个危险因素，而软性饮食喂养会导致记忆障碍。最近的研究表明，在幼龄动物模型中，脑源性神经营养因子（BDNF）与牙齿脱落或软性饮食有关，并且在阿尔茨海默病患者中BDNF表达降低。然而，牙齿脱落和/或软性饮食对大脑功能的单一或联合影响尚未完全了解。本实验研究了脱臼齿和粉饲对成年C57BL/6J小鼠记忆能力和海马BDNF mRNA表达的影响。将28周龄C57BL/6J小鼠分为完整磨牙组和拔牙组。随机分为I/S组（完整上磨牙/固体日粮喂养）、E/S组（拔出上磨牙/固体日粮喂养）、I/P组（完整上磨牙/粉末日粮喂养）和E/P组（拔出上磨牙/粉末日粮喂养）。观察期分别为4周和16周。采用渐进式被动回避测验分析记忆能力。实时聚合酶链反应（RT-PCR）分析海马bdnf相关mRNA表达。4周后，我们进行记忆测试和脑分离分析。四组大鼠记忆功能及BDNF mRNA水平无显著差异。16周后，E/S组和E/P组大鼠出现记忆障碍，BDNF mRNA水平下降。然而，即使在16周后，粉饲对记忆功能和BDNF mRNA水平也没有影响。由此可见，磨牙丢失和粉末日粮对记忆功能和BDNF mRNA水平的影响不同，磨牙丢失对记忆能力的长期影响可能大于粉末日粮。
It is known that tooth loss is known to be a risk factor for Alzheimer’s disease and soft diet feeding induces memory impairment. Recent studies have shown that brain-derived neurotrophic factor (BDNF) is associated with tooth loss or soft diet in young animal model, and that BDNF expression is decreased in patients with Alzheimer’s disease. However, single or combined effect of tooth loss and/or soft diet on brain function has not fully understood. Here we examined the effect of molar loss and powder diet on memory ability and the expression of BDNF mRNA in the hippocampus of adult C57BL/6J mice. Twenty eight-weeks-old C57BL/6J mice were divided into intact molar group and extracted molar group. They were randomly divided into the I/S group (Intact upper molar teeth/Solid diet feeding), the E/S group (Extracted upper molar teeth/Solid diet feeding), the I/P group (Intact upper molar teeth/Powder diet feeding), and the E/P group (Extracted upper molar teeth/Powder diet feeding). The observation periods were 4 and 16-week. To analyze the memory ability, the step-through passive avoidance test was conducted. BDNF-related mRNA in the hippocampus was analyzed by real-time polymerase chain reaction (RT-PCR). At 4 weeks later, we performed memory test and isolated brains to analyze. There were no differences in memory function and BDNF mRNA level between these four groups. However, at 16 weeks later, E/S and E/P group showed memory impairment, and decreased level of BDNF mRNA. Whereas, the powder diet had no effect on memory function and BDNF mRNA level even at 16 weeks later. These results suggest that the effect of molar loss and powder diet on memory function and BDNF mRNA levels were different, molar loss may have a greater long-term effect on memory ability than powder diet does.
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影响因子：
82.9
作者：
Eriksson, PS;Perfilieva, E;Gage, FH
通讯作者：
Gage, FH
影响因子：
4.2
作者：
Aberg, Fredrik;Helenius-Hietala, Jaana;Isoniemi, Helena
通讯作者：
Isoniemi, Helena
影响因子：
3.3
作者：
Brummelte, S.;Galea, L. A. M.
通讯作者：
Galea, L. A. M.
影响因子：
2.1
作者：
Bergdahl, Maud;Habib, Reza;Nilsson, Lars-Goran
通讯作者：
Nilsson, Lars-Goran
DOI：
10.1073/pnas.95.6.3168
发表时间：
1998-03-17
影响因子：
11.1
作者：
Gould, E;Tanapat, P;Fuchs, E
通讯作者：
Fuchs, E