Dynamic transcriptome analysis of NFAT family in guided bone regeneration with occlusive periosteum in swine model.
Dynamic transcriptome analysis of NFAT family in guided bone regeneration with occlusive periosteum in swine model.
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NFAT家族在猪骨膜闭塞引导骨再生中的动态转录组分析
DOI：
10.1186/s13018-022-03252-9
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发表时间：
2022-07-26
影响因子：
2.6
通讯作者：
中科院分区：
文献类型：
--
作者：
探讨引导性骨再生过程中骨膜NFAT家族的动态表达。以一侧猪肋骨为创伤组，对侧肋骨为对照组。切除肋骨段后，缝合骨膜形成一个封闭的空腔，模拟引导骨再生。在9个时间点收集骨膜和再生骨组织进行基因测序和苏木精-伊红染色。提取每个成员的表达数据进行分析。分析各成员间的表达相关性。术后1个月骨组织染色显示引导性骨再生基本完成，骨改建进入后期。两组各成员表达水平均发生较大变化，尤其是术后1.5个月内。创伤组NFATc 1和NFATC 2 IP的表达与对照组有显著相关性。各成员的倍数变化也有较大的波动，特别是在1.5个月内。创伤组NFATc 2、NFATc 4表达明显上调，且1个月内各时间点NFAT家族表达呈明显聚集相关，类似“模式-阻滞”现象。本研究揭示了NFAT家族在引导性骨再生中的动态表达，并为具体机制提供了参考。前1.5个月是一个关键时期，应予以重视。NFATc 2和NFATc 4的显著高表达可能在这一过程中发挥重要作用，这需要进一步的研究来验证。在线版本包含补充材料，可在10.1186/s13018-022-03252-9获得。
To investigate the dynamic expression of NFAT family of periosteum in guided bone regeneration process. The swine ribs on one side were used as the trauma group and the contralateral side as the control group. After rib segment was removed, periosteum was sutured to form a closed cavity mimicking guided bone regeneration. The periosteum and regenerated bone tissue were collected at nine time points for gene sequencing and hematoxylin–eosin staining. The expression data of each member were extracted for analysis. Expression correlations among various members were analyzed. Staining showed the guided bone regeneration was almost completed 1 month after the operation with later stage for bone remodeling. The expression levels of each member in both groups changed greatly, especially within postoperative 1.5 months. The expression of NFATc1 and NFATC2IP in trauma group was significantly correlated with those of control group. The foldchange of each member also had large fluctuations especially within 1.5 months. In the trauma group, NFATc2 and NFATc4 were significantly upregulated, and there was a significant aggregation correlation of NFAT family expression between the various time points within one month, similar to the “pattern-block” phenomenon. This study revealed the dynamic expression of NFAT family in guided bone regeneration, and provided a reference for the specific mechanism. The first 1.5 months is a critical period and should be paid attention to. The significant high-expression of NFATc2 and NFATc4 may role importantly in this process, which needs further research to verify it. The online version contains supplementary material available at 10.1186/s13018-022-03252-9.
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