Molecular evidence for the evolution of ichnoviruses from ascoviruses by symbiogenesis.
Molecular evidence for the evolution of ichnoviruses from ascoviruses by symbiogenesis.
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通过共生形成从Ascovires进化的分子证据。
DOI：
10.1186/1471-2148-8-253
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发表时间：
2008-09-18
影响因子：
3.4
通讯作者：
Federici BA
中科院分区：
文献类型：
--
作者：
Bigot Y;Samain S;Augé-Gouillou C;Federici BA
雌性内寄生姬蜂将病毒样颗粒注入毛毛虫宿主体内以抑制免疫力。这些颗粒被归类为丝状病毒病毒颗粒，类似于囊病毒病毒颗粒，它们也通过寄生黄蜂传播并攻击毛毛虫。子囊病毒复制 DNA 并产生病毒颗粒。多 DNA 病毒 DNA 由黄蜂 DNA 组成，由黄蜂从其基因组复制，也指导颗粒合成。囊病毒和丝状病毒颗粒之间的结构相似性以及它们传播的生物学表明丝状病毒是从囊状病毒进化而来的，尽管缺乏支持这一假设的分子证据。在这里，我们展示了 Glypta fumiferanae icnovirus 中的一个独特的 pox-D5 NTPase 蛋白家族与 ORF 90、91 和 93 编码的三种 Diadromus pulchellus 囊病毒蛋白相关。一种新的比对技术还表明，来自相关 ichnovirus 的两种蛋白是其他囊病毒病毒体蛋白的直系同源物。我们的研究结果提供了分子证据，支持通过将子囊病毒基因横向转移到姬蜂基因组中，从子囊病毒起源，这也许是大型DNA病毒和真核生物之间共生的第一个例子。我们还通过补充研究讨论了这一证据的局限性，这些研究表明，病毒基因在多DNA病毒、细菌和黄蜂基因组之间的被动横向转移可能通过进化过程中病毒基因组重组和复制的紧密耦合而反复发生。在共生理论的背景下考虑了被动横向转移对多DNA病毒和具有大双链基因组的病毒之间进化关系的影响。
Female endoparasitic ichneumonid wasps inject virus-like particles into their caterpillar hosts to suppress immunity. These particles are classified as ichnovirus virions and resemble ascovirus virions, which are also transmitted by parasitic wasps and attack caterpillars. Ascoviruses replicate DNA and produce virions. Polydnavirus DNA consists of wasp DNA replicated by the wasp from its genome, which also directs particle synthesis. Structural similarities between ascovirus and ichnovirus particles and the biology of their transmission suggest that ichnoviruses evolved from ascoviruses, although molecular evidence for this hypothesis is lacking. Here we show that a family of unique pox-D5 NTPase proteins in the Glypta fumiferanae ichnovirus are related to three Diadromus pulchellus ascovirus proteins encoded by ORFs 90, 91 and 93. A new alignment technique also shows that two proteins from a related ichnovirus are orthologs of other ascovirus virion proteins. Our results provide molecular evidence supporting the origin of ichnoviruses from ascoviruses by lateral transfer of ascoviral genes into ichneumonid wasp genomes, perhaps the first example of symbiogenesis between large DNA viruses and eukaryotic organisms. We also discuss the limits of this evidence through complementary studies, which revealed that passive lateral transfer of viral genes among polydnaviral, bacterial, and wasp genomes may have occurred repeatedly through an intimate coupling of both recombination and replication of viral genomes during evolution. The impact of passive lateral transfers on evolutionary relationships between polydnaviruses and viruses with large double-stranded genomes is considered in the context of the theory of symbiogenesis.
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影响因子：
1.4
作者：
AVERY S W;JOUVENAZ D P;ANTHONY D W
通讯作者：
ANTHONY D W
影响因子：
3.4
作者：
HESS, RT;BENHAM, GS;POINAR, GO
通讯作者：
POINAR, GO
影响因子：
3.7
作者：
Deng, LQ;Stoltz, DB;Webb, BA
通讯作者：
Webb, BA
影响因子：
1.7
作者：
HAMM, JJ;NORDLUNG, DA;MARTI, OG
通讯作者：
MARTI, OG
影响因子：
10.7
作者：
Hughes, AL;Friedman, R
通讯作者：
Friedman, R