A mutation in LacDWARF1 results in a GA-deficient dwarf phenotype in sponge gourd (Luffa acutangula).
A mutation in LacDWARF1 results in a GA-deficient dwarf phenotype in sponge gourd (Luffa acutangula).
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LacDWARF1 的突变导致丝瓜 (Luffa acutangula) 出现 GA 缺陷的矮化表型。
DOI：
10.1007/s00122-021-03938-4
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发表时间：
2021-10
期刊：
影响因子：
--
通讯作者：
Wu H
中科院分区：
文献类型：
--
作者：
Zhao G;Luo C;Luo J;Li J;Gong H;Zheng X;Liu X;Guo J;Zhou L;Wu H
通过结合BSA-Seq和比较基因组学分析，将矮秆基因LacDWARF 1定位于05号染色体上65.4kb的物理基因组区域。矮秆性状是葫芦科育种中最重要的性状之一，因为它节省了劳动力，提高了收获指数。据我们所知，以前没有关于丝瓜侏儒症的研究。本研究报道了第一个矮化突变体WJ 209，其细胞大小和节间减小。遗传分析表明，该突变体的表型受一对隐性基因控制，命名为Lacdwarf 1（Lacd 1）。结合群体分离分析和新一代测序技术，我们利用F2分离群体用InDel和SNP多态性标记快速定位了5号染色体上一个65.4kb的区域。基因注释显示Lac 05 g 019500编码赤霉素3β-羟化酶（GA 3 ox），其功能最可能是Lacd 1的候选基因。DNA序列分析表明，WJ 209中Lac 05 g 019500的第一个内含子中存在约4kb的插入片段。Lac 05 g 019500在矮突变体中由于插入的存在而不正确地转录。此外，WJ 209的生物活性GA显著降低，外源GA 3处理可恢复其矮化表型，表明WJ 209为GA缺陷型突变体。这些结果支持Lac 05 g 019500为Lacd 1基因的结论。此外，RNA-Seq结果显示，与野生型相比，WJ 209中的许多基因，包括与植物激素、细胞过程、细胞壁、膜和胁迫响应相关的基因，都发生了显著改变。本研究为分子标记辅助育种在矮生丝瓜上的应用提供了依据。在线版本包含补充材料，可在（10.1007/s 00122 -021-03938-4）上获得。
A dwarfism gene LacDWARF1 was mapped by combined BSA-Seq and comparative genomics analyses to a 65.4 kb physical genomic region on chromosome 05. Dwarf architecture is one of the most important traits utilized in Cucurbitaceae breeding because it saves labor and increases the harvest index. To our knowledge, there has been no prior research about dwarfism in the sponge gourd. This study reports the first dwarf mutant WJ209 with a decrease in cell size and internodes. A genetic analysis revealed that the mutant phenotype was controlled by a single recessive gene, which is designated Lacdwarf1 (Lacd1). Combined with bulked segregate analysis and next-generation sequencing, we quickly mapped a 65.4 kb region on chromosome 5 using F2 segregation population with InDel and SNP polymorphism markers. Gene annotation revealed that Lac05g019500 encodes a gibberellin 3β-hydroxylase (GA3ox) that functions as the most likely candidate gene for Lacd1. DNA sequence analysis showed that there is an approximately 4 kb insertion in the first intron of Lac05g019500 in WJ209. Lac05g019500 is transcribed incorrectly in the dwarf mutant owing to the presence of the insertion. Moreover, the bioactive GAs decreased significantly in WJ209, and the dwarf phenotype could be restored by exogenous GA3 treatment, indicating that WJ209 is a GA-deficient mutant. All these results support the conclusion that Lac05g019500 is the Lacd1 gene. In addition, RNA-Seq revealed that many genes, including those related to plant hormones, cellular process, cell wall, membrane and response to stress, were significantly altered in WJ209 compared with the wild type. This study will aid in the use of molecular marker-assisted breeding in the dwarf sponge gourd. The online version contains supplementary material available at (10.1007/s00122-021-03938-4).
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