Confinement-induced quorum sensing of individual Staphylococcus aureus bacteria.
Confinement-induced quorum sensing of individual Staphylococcus aureus bacteria.
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DOI：
10.1038/nchembio.264
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发表时间：
2010-01
影响因子：
14.8
通讯作者：
Brinker CJ
中科院分区：
文献类型：
--
作者：
Carnes EC;Lopez DM;Donegan NP;Cheung A;Gresham H;Timmins GS;Brinker CJ
据推测，除了细胞密度，其他因素，如环境的尺寸和扩散特性，可以影响群体感应（QS）和诱导的遗传重编程。建模研究预测，QS可以在单个细胞的水平上运行，但是，由于实验挑战，单个细胞的QS的潜在益处实际上仍未被探索。在这里，我们报告了一个物理系统，模拟细菌的隔离，如在感染过程中的内体或吞噬体内，并在完全化学和物理隔离的条件下保持细胞活力。对于金黄色葡萄球菌，我们表明群体感应和遗传重编程发生在一个单一的孤立的有机体。群体感应允许S.金黄色葡萄球菌，以感觉限制和激活生存所需的毒力和代谢途径。为了证明限制诱导的群体感应对个体的益处，我们显示群体感应细菌比非QS细菌具有显著更大的生存力。
It is postulated that, in addition to cell density, other factors, such as the dimensions and diffusional characteristics of the environment, could influence quorum sensing (QS) and induction of genetic reprogramming. Modeling studies predict that QS may operate at the level of a single cell, but, due to experimental challenges, the potential benefits of QS by individual cells remain virtually unexplored. Here we report a physical system that mimics isolation of a bacterium, such as within an endosome or phagosome during infection, and maintains cell viability under conditions of complete chemical and physical isolation. For Staphylococcus aureus, we show quorum sensing and genetic re-programming to occur in a single isolated organism. Quorum sensing allows S. aureus to sense confinement and to activate virulence and metabolic pathways needed for survival. To demonstrate the benefit of confinement-induced quorum sensing to individuals, we showed quorum sensing bacteria to have significantly greater viability over non-QS bacteria.
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