Characterization of the arginolytic microflora provides insights into pH homeostasis in human oral biofilms.
Characterization of the arginolytic microflora provides insights into pH homeostasis in human oral biofilms.
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DOI：
10.1159/000365296
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发表时间：
2015
期刊：
影响因子：
4.2
通讯作者：
Nascimento MM
中科院分区：
文献类型：
--
作者：
Huang X;Schulte RM;Burne RA;Nascimento MM
通常与牙齿健康相关的一组选定的口腔细菌能够通过精氨酸脱亚胺酶系统（ADS）产生碱，这对人类口腔生物膜的pH值具有深远的影响。龋齿风险的增加与口腔生物膜中细菌ADS活性的降低有关。从无龋（CF）和龋活跃（CA）成人的牙菌斑样本中分离精氨酸溶解细菌菌株，并进行表征，以研究个体间牙菌斑ADS活性差异的基础。通过16 S rRNA基因测序鉴定了五十六株ADS阳性细菌菌株，并在标准生长条件下比较了它们的ADS活性水平。细菌ADS活性范围为45.2至688.0单位（mg蛋白）-1。虽然血链球菌是最常见的菌种，但也有其他链球菌。在已知诱导或抑制ADS基因表达的条件下，使用27种ADS阳性菌株进行的生化测定显示，响应于pH值、氧气和碳水化合物或精氨酸的可用性，溶血活性存在实质性变化。这项研究表明，临床菌株中观察到的广谱溶血性表达的基础至少部分归因于物种内和物种间ADS调控的差异。这些结果为口腔生物膜中ADS活性的受试者间差异提供了微生物学基础，并增强了我们对龋齿作为一种生态驱动的疾病的理解，在这种疾病中，精氨酸代谢调节菌斑pH值并促进牙齿健康。
A selected group of oral bacteria commonly associated with dental health is capable of producing alkali via the arginine deiminase system (ADS), which has a profound impact on the pH of human oral biofilms. An increased risk for dental caries has been associated with reduced ADS activity of the bacteria in oral biofilms. Arginolytic bacterial strains from dental plaque samples of caries-free (CF) and caries-active (CA) adults were isolated and characterized to investigate the basis for differences in plaque ADS activity between individuals. Fifty-six ADS-positive bacterial strains were identified by 16S rRNA gene sequencing and their ADS activity levels were compared under standard growth conditions. The spectrum of bacterial ADS activity ranged from 45.2 to 688.0 units (mg protein)−1. Although Streptococcus sanguinis was the most prevalent species, other Streptococcus were also represented. Biochemical assays carried out using twenty-seven ADS-positive strains under conditions known to induce or repress ADS gene expression, showed substantial variation in arginolytic activity in response to pH, oxygen, and the availability of carbohydrate or arginine. This study reveals that the basis for the wide spectrum of arginolytic expression observed among clinical strains is, at least in part, attributable to differences in the regulation of the ADS within and between species. The results provide insights into the microbiological basis for inter-subject differences in ADS activity in oral biofilms and enhance our understanding of dental caries as an ecologically-driven disease in which arginine metabolism moderates plaque pH and promotes dental health.
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影响因子：
3.2
作者：
Dong, YQ;Chen, YYM;Burne, RA
通讯作者：
Burne, RA
影响因子：
3
作者：
KLEINBERG, I;JENKINS, GN
通讯作者：
JENKINS, GN
影响因子：
7.6
作者：
MARGOLIS, HC;DUCKWORTH, JH;MORENO, EC
通讯作者：
MORENO, EC
影响因子：
7.6
作者：
Nascimento, M. M.;Liu, Y.;Burne, R. A.
通讯作者：
Burne, R. A.
影响因子：
3.2
作者：
Burne, RA;Wen, ZT;Penders, JEC
通讯作者：
Penders, JEC