Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation
Comparison of bacterial community characteristics between complete and shortcut denitrification systems for quinoline degradation
复制标题
喹啉降解完全反硝化系统与短程反硝化系统细菌群落特征比较
DOI：
10.1007/s00253-016-7949-y
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发表时间：
2016-11
影响因子：
5
通讯作者：
Yue Xiuping
中科院分区：
文献类型：
--
作者：
Zhang Xiaomei;Hua Xiufu;Yue Xiuping
对于喹啉类化合物的快速反硝化降解，研究较少。本研究以喹啉为降解对象，同时构建了完整和短程反硝化系统。通过计算，完全系统和短程系统的喹啉比去除率分别为0.905和1.123 g/（gVSS d），短程系统是完全系统的1.24倍。采用聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）、高通量测序和基于16 SrRNA的定量PCR（qPCR）技术，对两种系统的微生物群落结构进行比较。在两个系统中均检测到大量的微生物门、纲、属。变形菌门、γ变形菌纲和嗜脂环菌属是喹啉降解的主要贡献者。与完全反硝化系统相比，短程反硝化系统中起关键作用的主要和特异性菌株较多，物种丰富度和功能基因（narG、nirS、nirK和nosZ）的总丰度较高。可以推测，短程反硝化系统中细菌群落的无机氮还原酶活性较强，这是导致反硝化速率较高的内在原因。
For quinoline-denitrifying degradation, very few researches focused on shortcut denitrification process and its bacterial community characteristics. In this study, complete and shortcut denitrification systems were constructed simultaneously for quinoline degradation. By calculation, specific quinoline removal rates were 0.905 and 1.123 g/(gVSS d), respectively, in the complete and shortcut systems, and the latter was 1.24 times of the former. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), high-throughput sequencing, and quantitative PCR (qPCR) techniques based on 16S rRNA were jointly applied to compare microbial community structures of two systems. Many denitrifying bacteria phyla, classes, and genera were detected in the two systems. Phylum Proteobacteria, Class Gammaproteobacteria, and GenusAlicycliphilus denitrificanswere the dominant contributors for quinoline-denitrifying degradation. In the shortcut denitrification system, main and specific strains playing crucial roles were more; the species richness and the total abundance of functional genes (narG,nirS,nirK, andnosZ) were higher compared with the complete denitrification system. It could be supposed that inorganic-nitrogen reductase activity of bacterial community was stronger in the shortcut denitrification system, which was the intrinsic reason to result in higher denitrification rate.
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影响因子：
11.4
作者：
Qinghua Sun;Yaohui Bai;Cui Zhao;Ya-na Xiao;Donghui Wen;Xiao-yan Tang
通讯作者：
Qinghua Sun;Yaohui Bai;Cui Zhao;Ya-na Xiao;Donghui Wen;Xiao-yan Tang
DOI：
--
发表时间：
2010
期刊：
Microbiology, China
影响因子：
--
作者：
Zhong Hui
通讯作者：
Zhong Hui
影响因子：
12.8
作者：
Garcia-Lledo, Arantzazu;Vilar-Sanz, Ariadna;Baneras, Lluis
通讯作者：
Baneras, Lluis
影响因子：
3.8
作者：
Cortes-Lorenzo, C.;Sipkema, D.;Gonzalez-Lopez, J.
通讯作者：
Gonzalez-Lopez, J.
影响因子：
8.8
作者：
Hua, Xiufu;Wu, Zuojun;Liu, Zheng
通讯作者：
Liu, Zheng