Operating Cooperatively (OC) sensor for highly specific recognition of nucleic acids.
Operating Cooperatively (OC) sensor for highly specific recognition of nucleic acids.
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DOI：
10.1371/journal.pone.0055919
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Kolpashchikov DM
中科院分区：
文献类型：
--
作者：
Cornett EM;O'Steen MR;Kolpashchikov DM
Molecular Beacon (MB) probes have been extensively used for nucleic acid analysis because of their ability to produce fluorescent signal in solution instantly after hybridization. The indirect binding of MB probe to a target analyte offers several advantages, including: improved genotyping accuracy and the possibility to analyse folded nucleic acids. Here we report on a new design for MB-based sensor, called ‘Operating Cooperatively’ (OC), which takes advantage of indirect binding of MB probe to a target analyte. The sensor consists of two unmodified DNA strands, which hybridize to a universal MB probe and a nucleic acid analyte to form a fluorescent complex. OC sensors were designed to analyze two human SNPs and E.coli 16S rRNA. High specificity of the approach was demonstrated by the detection of true analyte in over 100 times excess amount of single base substituted analytes. Taking into account the flexibility in the design and the simplicity in optimization, we conclude that OC sensors may become versatile and efficient tools for instant DNA and RNA analysis in homogeneous solution.
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影响因子：
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Kolpashchikov, Dmitry M
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