Ca(2+)-transporting ATPase, phospholamban, and calsequestrin levels in nonfailing and failing human myocardium.
Ca(2+)-transporting ATPase, phospholamban, and calsequestrin levels in nonfailing and failing human myocardium.
复制标题
非衰竭和衰竭人类心肌中 Ca(2 ) 转运 ATP 酶、受磷蛋白和钙螯合蛋白水平。
DOI：
10.1161/01.cir.90.2.653
复制
发表时间：
1994
期刊：
影响因子：
37.8
通讯作者：
Jones,LR
中科院分区：
文献类型：
--
作者：
Movsesian,MA;Karimi,M;Green,K;Jones,LR
背景：在衰竭的人左室心肌中观察到细胞内钙瞬变的舒张期成分异常，增加了参与钙转运的肌浆网蛋白水平或功能降低的可能性，这与人类扩张型心肌病的病理生理有关。然而，功能分析表明，从正常和衰竭的人左室心肌制备的肌浆网丰富的微粒体中，ATP依赖的钙转运或磷蛋白对其调节没有差异。本研究的目的是定量测定无衰竭和衰竭的人左室心肌中钙离子直接转运的ATPase、磷蛋白和钙库蛋白的蛋白水平。方法和结果分别从6例左心室收缩功能正常的非匹配器官供者的非衰竭左室心肌和6例因特发性扩张型心肌病所致的IV级心力衰竭的移植受者的切除心脏中制备了非衰竭左室心肌和衰竭左室心肌的蛋白质提取物。用单抗和亲和纯化的多克隆抗体进行定量免疫印迹，测定钙转运ATPase、磷蛋白和钙调素的含量。结论本研究结果表明，在衰竭的人左室心肌中，钙离子转运的ATPase、磷蛋白和钙化蛋白的蛋白水平并未降低，钙离子转运的ATPase和磷蛋白的下调不是扩张型心肌病分子病理生理机制的一部分。
BACKGROUNDObservations of abnormalities in the diastolic components of intracellular Ca2+ transients in failing human left ventricular myocardium have raised the possibility that reductions in the level or function of sarcoplasmic reticulum proteins involved in Ca2+ transport contribute to the pathophysiology of dilated cardiomyopathy in humans. Functional assays, however, have revealed no differences in ATP-dependent Ca2+ transport or its modulation by phospholamban in sarcoplasmic reticulum-enriched microsomes prepared from nonfailing and failing human left ventricular myocardium. The purpose of the present study was to quantify protein levels of Ca(2+)-transporting ATPase, phospholamban, and calsequestrin directly in nonfailing and failing human left ventricular myocardium.METHOD AND RESULTSTotal protein extracts were prepared from nonfailing left ventricular myocardium from the hearts of unmatched organ donors with normal left ventricular contractility (n = 6) and from failing left ventricular myocardium from the excised hearts of transplant recipients with class IV heart failure resulting from idiopathic dilated cardiomyopathy (n = 6). Ca(2+)-transporting ATPase, phospholamban, and calsequestrin contents were determined by quantitative immunoblotting with monoclonal and affinity-purified polyclonal antibodies. The levels of the three proteins were identical in nonfailing and failing human left ventricular myocardium.CONCLUSIONSThese results indicate that protein levels of Ca(2+)-transporting ATPase, phospholamban, and calsequestrin are not diminished in failing human left ventricular myocardium and that downregulation of the Ca(2+)-transporting ATPase and phospholamban is not part of the molecular pathophysiology of dilated cardiomyopathy in humans.
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影响因子：
10.8
作者：
N. Staley;G. Noren;S. Einzig;T. Rublein
通讯作者：
T. Rublein
影响因子：
20.1
作者：
ARAI, M;ALPERT, NR;PERIASAMY, M
通讯作者：
PERIASAMY, M
特发性扩张型心肌病患者心脏肌浆网对 Ca2 的摄取。
影响因子：
20.1
作者：
Movsesian,MA;Bristow,MR;Krall,J
通讯作者：
Krall,J
DOI：
10.1152/ajpcell.1993.264.5.c1308
发表时间：
1993-05
期刊：
The American journal of physiology
影响因子：
--
作者：
L. Schulte;J. Navarro;S. Kandarian
通讯作者：
L. Schulte;J. Navarro;S. Kandarian
DOI：
10.1073/pnas.91.7.2694
发表时间：
1994-03-29
影响因子：
11.1
作者：
ROCKMAN, HA;ONO, S;CHIEN, KR
通讯作者：
CHIEN, KR