A spider web strategy of type IV pili-mediated migration to build a fibre-like Psl polysaccharide matrix in Pseudomonas aeruginosa biofilms.
A spider web strategy of type IV pili-mediated migration to build a fibre-like Psl polysaccharide matrix in Pseudomonas aeruginosa biofilms.
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DOI：
10.1111/1462-2920.12095
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发表时间：
2013-08
影响因子：
5.1
通讯作者：
Ma LZ
中科院分区：
文献类型：
--
作者：
Wang S;Parsek MR;Wozniak DJ;Ma LZ
细菌运动性参与生物膜的形成。然而，尚不清楚细菌运动性如何/是否影响生物膜基质的形成。Psl多糖是铜绿假单胞菌的关键生物膜基质组分。在这里，我们报告说，IV型皮利（T4 P）介导的细菌迁移导致形成的纤维样Psl矩阵。野生型和鞭毛缺陷型菌株中T4 P的缺失导致Psl纤维的损失和流动池生物膜中生物膜生物量的减少以及空气-液体界面处的薄膜。缺乏T4 P驱动的抽搐运动性的细菌，包括那些仍然表达表面T4 P的细菌，不能形成Psl纤维。PSL-纤维基质的形成对于有效的生物膜形成是关键的，但不需要鞭毛和多糖Pel或藻酸盐。Psl纤维可能是由T4 P介导的迁移过程中细菌释放的Psl形成的，这是一种类似于蜘蛛网形成的策略。饥饿可以耦合Psl释放和T4 P驱动的抽搐运动。此外，径向图案PSL纤维基质存在于生物膜的中间，即营养缺乏区域。这意味着一个合理的模型，细菌如何响应营养有限的局部环境，建立一个多糖纤维基质的T4 P依赖性，细菌迁移策略。这种策略可能对自然和临床环境中的细菌存活具有普遍意义。
Bacterial motilities participate in biofilm development. However, it is unknown how/if bacterial motility affects formation of the biofilm matrix. Psl polysaccharide is a key biofilm matrix component of Pseudomonas aeruginosa. Here we report that type IV pili (T4P)-mediated bacterial migration leads to the formation of a fibre-like Psl matrix. Deletion of T4P in wild type and flagella-deficient strains results in loss of the Psl-fibres and reduction of biofilm biomass in flow cell biofilms as well as pellicles at air-liquid interface. Bacteria lacking T4P-driven twitching motility including those that still express surface T4P are unable to form the Psl-fibres. Formation of a Psl-fibre matrix is critical for efficient biofilm formation, yet does not require flagella and polysaccharide Pel or alginate. The Psl-fibres are likely formed by Psl released from bacteria during T4P-mediated migration, a strategy similar to spider web formation. Starvation can couple Psl release and T4P-driven twitching motility. Furthermore, a radial-pattern Psl-fibre matrix is present in the middle of biofilms, a nutrient-deprived region. These imply a plausible model for how bacteria respond to nutrient-limited local environment to build a polysaccharide-fibre matrix by T4P-dependent, bacterial migration strategy. This strategy may have general significance for bacterial survival in natural and clinical settings.
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影响因子：
3.1
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