Femtosecond laser ablation enhances cell infiltration into three-dimensional electrospun scaffolds.
Femtosecond laser ablation enhances cell infiltration into three-dimensional electrospun scaffolds.
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DOI：
10.1016/j.actbio.2012.04.023
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发表时间：
2012-07
影响因子：
9.7
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Lee BL;Jeon H;Wang A;Yan Z;Yu J;Grigoropoulos C;Li S
静电纺丝支架广泛用于组织工程应用，因为它们提供了一个细胞友好的微环境。然而，一个主要的限制是致密的纤维、小的孔径和因此差的细胞浸润。在这里，我们采用飞秒（FS）激光系统烧蚀和静电纺丝聚（L-丙交酯）（PLLA）纳米纤维支架上创建微尺度功能。在确定烧蚀参数后，我们在支架上图案化不同直径的50 μ m、100 μm和200 μ m的结构孔，相邻孔之间的间距为50 μm和200 μm。烧蚀后支架的弹性模量随着间距的减小和孔径的增大而减小。与对照（未消融）支架相比，接种在激光消融支架上的细胞表现出不同的形态，但增殖率相似。此外，动物研究表明，消融支架促进内皮细胞向内生长，以及急剧增加M2巨噬细胞和整体细胞浸润。这些发现表明，FS激光消融可用于增加细胞浸润到纳米纤维支架中。激光消融不仅可以在微米尺度上形成所需的结构，而且为组织工程三维多孔结构的制备提供了一种新的方法。
Electrospun scaffolds are used extensively in tissue engineering applications since they offer a cell-friendly microenvironment. However, one major limitation is the dense fibers, small pore size and consequently poor cell infiltration. Here, we employ a femtosecond (FS) laser system to ablate and create microscale features on electrospun poly(L-lactide) (PLLA) nanofibrous scaffolds. Upon determining the ablation parameters, we pattern structured holes of varying diameters of 50, 100, and 200 μm and spacing of 50 and 200 μm between adjacent holes on the scaffolds. The elastic moduli of ablated scaffolds decrease with the decrease of spacing and the increase of hole size. Cells seeded on the laser-ablated scaffolds exhibit different morphology but similar proliferation rate when compared with control (non-ablated) scaffold. Furthermore, animal studies indicate that ablated scaffolds facilitate endothelial cell ingrowth as well as drastically increase M2 macrophage and overall cell infiltration. These findings demonstrate that FS laser ablation can be used to increase cell infiltration into nanofibrous scaffolds. Laser ablation not only can create desired features in micrometer length scale but also presents a new approach in the fabrication of three-dimensional porous constructs for tissue engineering.
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影响因子：
14
作者：
Park, Jennifer S.;Chu, Julia S.;Tsou, Anchi D.;Diop, Rokhaya;Tang, Zhenyu;Wang, Aijun;Li, Song
通讯作者：
Li, Song
影响因子：
6.2
作者：
Galperin, Anna;Long, Thomas J.;Ratner, Buddy D.
通讯作者：
Ratner, Buddy D.
DOI：
10.1073/pnas.0704581104
发表时间：
2007-07-17
影响因子：
11.1
作者：
Hashi, Craig K.;Zhu, Yiqian;Li, Song
通讯作者：
Li, Song
影响因子：
4.6
作者：
Tan, SH;Inai, R;Ramakrishna, S
通讯作者：
Ramakrishna, S
影响因子：
--
作者：
Ma, ZW;Kotaki, M;Ramakrishna, S
通讯作者：
Ramakrishna, S