4-Hydroxybenzaldehyde accelerates acute wound healing through activation of focal adhesion signalling in keratinocytes.
4-Hydroxybenzaldehyde accelerates acute wound healing through activation of focal adhesion signalling in keratinocytes.
复制标题
DOI：
10.1038/s41598-017-14368-y
复制
发表时间：
2017-10-27
影响因子：
4.6
通讯作者：
Lee EJ
中科院分区：
文献类型：
--
作者：
Kang CW;Han YE;Kim J;Oh JH;Cho YH;Lee EJ
4-羟基苯甲醛（4-HBA）是天然存在的苯甲醛，是天麻的主要活性成分.虽然最近的研究已经证明了4-HBA的代谢作用，但对4-HBA在急性伤口愈合中的生理作用知之甚少。本研究旨在探讨4-HBA对急性创伤愈合的影响及其机制。使用体外方法，我们发现4-HBA通过增加粘着斑激酶和Src活性显著促进角质形成细胞的迁移和侵袭。此外，4-HBA治疗还促进了体内切除伤口动物模型中的伤口愈合和上皮再形成。4-HBA与血小板衍生生长因子亚基B同源二聚体联合治疗在促进伤口愈合方面显示出协同作用。总之，我们的结果表明，4-HBA治疗促进角质形成细胞迁移和伤口愈合的小鼠皮肤通过Src/丝裂原活化蛋白激酶途径。因此，4-HBA可能是具有促进急性伤口愈合潜力的候选治疗剂。
4-Hydroxybenzaldehyde (4-HBA) is a naturally occurring benzaldehyde and the major active constituent of Gastrodia elata. While recent studies have demonstrated metabolic effects of 4-HBA, little is known about the physiological role of 4-HBA in acute wound healing. Here, we investigated the effects and mechanisms of 4-HBA on acute wound healing. Using an in vitro approach, we found that 4-HBA significantly promoted keratinocyte cell migration and invasion by increasing focal adhesion kinase and Src activity. In addition, 4-HBA treatment also promoted wound healing and re-epithelialization in an in vivo excision wound animal model. Combination treatment with 4-HBA and platelet-derived growth factor subunit B homodimer showed synergistic effects in promoting wound healing. Taken together, our results demonstrated that treatment with 4-HBA promoted keratinocyte migration and wound healing in mouse skin through the Src/mitogen-activated protein kinase pathway. Therefore, 4-HBA could be a candidate therapeutic agent with the potential to promote acute wound healing.
登录
查看更多内容
影响因子：
16.6
作者：
Miao, Qi;Ku, Amy T.;Nishino, Yudai;Howard, Jeffrey M.;Rao, Ajay S.;Shaver, Timothy M.;Garcia, Gloria E.;Le, Diep N.;Karlin, Kristen L.;Westbrook, Thomas F.;Poli, Valeria;Nguyen, Hoang
通讯作者：
Nguyen, Hoang
影响因子：
0.9
作者：
Bauer, Stephen M;Bauer, Richard J;Velazquez, Omaida C
通讯作者：
Velazquez, Omaida C
影响因子：
5
作者：
Park, Sunmin;Kim, Da Sol;Kang, Suna
通讯作者：
Kang, Suna
影响因子：
5.3
作者：
Tan, Nguan Soon;Icre, Guillaume;Michalik, Liliane
通讯作者：
Michalik, Liliane
影响因子：
6.5
作者：
Repertinger, SK;Campagnaro, E;Hansen, LA
通讯作者：
Hansen, LA