A study of carbobenzoxy-D-phenylalanine-L-phenylalanine-glycine, an inhibitor of membrane fusion, in phospholipid bilayers with multinuclear magnetic resonance.
A study of carbobenzoxy-D-phenylalanine-L-phenylalanine-glycine, an inhibitor of membrane fusion, in phospholipid bilayers with multinuclear magnetic resonance.
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利用多核磁共振研究磷脂双层中的苯甲氧基-D-苯丙氨酸-L-苯丙氨酸-甘氨酸（一种膜融合抑制剂）。
DOI：
10.1016/0005-2736(95)80007-3
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Yeagle,PL
中科院分区：
文献类型：
--
作者：
Dentino,AR;Westerman,PW;Yeagle,PL
在磷脂双层中研究了抗病毒和膜融合抑制剂苄氧羰基-d-苯丙氨酸-l-苯丙氨酸-甘氨酸（ZfFG），早期的研究表明该肽起作用。使用同位素标记的肽进行多核磁共振（NMR）研究。合成了一种在甘氨酸羧基上用13 C标记的肽，并测定了该碳的各向同性13 C-NMR化学位移随pH的变化。从与磷脂酰胆碱双层结合的肽中测定了羧基的pKa为3.6。ZfFG通过超声处理抑制高度弯曲的小单层囊泡的形成。作为pH函数的实验表明，ZfFG的这种能力受pKa为3.7的控制。因此，ZfFG的羧基的质子化状态似乎调节这种抗病毒肽在使高度弯曲的磷脂组装体不稳定方面的有效性。先前已经发现这种去稳定化与该肽的抗融合和抗病毒活性的机制有关。利用ZfFG的甘氨酸中羧基碳（富含13 C）的13 C自旋晶格弛豫的顺磁弛豫增强来探测ZfFG的羧基在膜中的位置。结果表明，该羧基位于磷脂双层的表面或表面以上。用2 H-NMR研究了甘氨酸α-碳质子中氘代的ZfFG在膜中的分子动力学。当ZfFG被绑定到膜的磷脂酰胆碱，sharp 2 H-NMR光谱成分被观察到，一致的肽的甘氨酸亚甲基部分的无序。当ZfFG在低于30° C的温度下与N-甲基二油酰磷脂酰乙醇胺（N-甲基DOPE）双层结合时，观察到大的四极分裂。这些结果表明，ZfFG可能抑制膜融合从表面的脂质双层，但不是通过形成一个紧密的，化学计量复合物与磷脂。
The anti-viral and membrane fusion inhibitor, carbobenzoxy-d-phenylalanine-l-phenylalanine-glycine (ZfFG), was studied in phospholipid bilayers, where earlier studies had indicated this peptide functioned. Multinuclear magnetic resonance (NMR) studies were performed with isotopically labeled peptide. A peptide labeled in the glycine carboxyl with13C was synthesized, and the isotropic13C-NMR chemical shift of that carbon was measured as a function of pH. A pKaof 3.6 for the carboxyl was determined from the peptide bound to a phosphatidylcholine bilayer. ZfFG inhibits the formation by sonication of highly curved, small unilamellar vesicles. Experiments as a function of pH revealed that this ability of ZfFG was governed by a pKaof 3.7. Therefore the protonation state of the carboxyl of ZfFG appeared to regulate the effectiveness of this anti-viral peptide at destabilizing highly curved phospholipid assemblies. Such destabilization had previously been discovered to be related to the mechanism of the anti-fusion and anti-viral activity of this peptide. The location of the carboxyl of ZfFG in the membrane was probed with paramagnetic relaxation enhancement of the13C spin lattice relaxation of the carboxyl carbon in the glycine of ZfFG (enriched in13C). Results suggested that this carboxyl is at or above the surface of the phospholipid bilayer. The dynamics of the molecule in the membrane were examined with2H-NMR studies of ZfFG, deuterated in the α-carbon protons of the glycine. When ZfFG was bound to membranes of phosphatidylcholine, a sharp2H-NMR spectral component was observed, consistent with a disordering of the glycine methylene segment of the peptide. When ZfFG was bound to N-methyl dioleoylphosphatidylethanolamine (N-methyl DOPE) bilayers at temperatures below 30° C, a large quadrupole splitting was observed. These results suggest that ZfFG likely inhibits membrane fusion from the surface of the lipid bilayer, but not by forming a tight, stoichiometric complex with the phospholipids.
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麻疹病毒-P 物质受体相互作用。
DOI：
10.1172/jci114571
发表时间：
1990
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Harrowe,G;Mitsuhashi,M;Payan,DG
通讯作者：
Payan,DG
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影响因子：
4
作者：
R. Epand
通讯作者：
R. Epand
含有 N-甲基化磷脂酰乙醇胺的双层膜的物理性质和表面相互作用。
影响因子：
2.9
作者：
Gagné,J;Stamatatos,L;Diacovo,T;Hui,SW;Yeagle,PL;Silvius,JR
通讯作者：
Silvius,JR
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影响因子：
64.8
作者：
P. Kroon;M. Kainosho;S. Chan
通讯作者：
S. Chan
DOI：
10.1073/pnas.72.4.1451
发表时间：
1975
影响因子：
11.1
作者：
P. Brûlet;H. Mcconnell
通讯作者：
H. Mcconnell