Electrochemically active bacteria sense electrode potentials for regulating catabolic pathways.
Electrochemically active bacteria sense electrode potentials for regulating catabolic pathways.
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DOI：
10.1038/s41467-018-03416-4
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发表时间：
2018-03-14
影响因子：
16.6
通讯作者：
Kouzuma A
中科院分区：
文献类型：
--
作者：
Hirose A;Kasai T;Aoki M;Umemura T;Watanabe K;Kouzuma A
电化学活性细菌（EAB）因其在生物电化学过程中的应用而受到广泛关注。虽然已知电极电位会影响EAB的代谢活性，但目前尚不清楚EAB是否能够感知和响应电极电位。在这里，我们发现，在高电位电极存在下，EAB希瓦氏菌MR-1模型可以利用nadh依赖的分解代谢途径和背景甲酸依赖的途径来实现高生长产量。我们还发现Arc调控系统参与了电极电位的感知和分解代谢基因的表达，包括NADH脱氢酶的基因。我们认为这些发现可以促进EAB在生物技术过程中的应用，并为其在自然栖息地的生态策略提供分子基础。电化学活性细菌（EAB）是否能根据电极电位获得能量尚不清楚。在这里，作者通过转录组和缺失突变体分析表明，EAB可以通过Arc系统感知电极电位，并激活nadh依赖的分解代谢途径来产生ATP。
Electrochemically active bacteria (EAB) receive considerable attention for their utility in bioelectrochemical processes. Although electrode potentials are known to affect the metabolic activity of EAB, it is unclear whether EAB are able to sense and respond to electrode potentials. Here, we show that, in the presence of a high-potential electrode, a model EAB Shewanella oneidensis MR-1 can utilize NADH-dependent catabolic pathways and a background formate-dependent pathway to achieve high growth yield. We also show that an Arc regulatory system is involved in sensing electrode potentials and regulating the expression of catabolic genes, including those for NADH dehydrogenase. We suggest that these findings may facilitate the use of EAB in biotechnological processes and offer the molecular bases for their ecological strategies in natural habitats. Whether electrochemically active bacteria (EAB) can gain energy according to electrode potentials is still unclear. Here, the authors show through transcriptome and deletion mutant analyses that EAB can sense electrode potentials by the Arc system and activate NADH-dependent catabolic pathway to generate ATP.
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影响因子：
4.4
作者：
Gao H;Wang X;Yang ZK;Palzkill T;Zhou J
通讯作者：
Zhou J
影响因子：
11
作者：
Ishii, Shun'ichi;Suzuki, Shino;Bretschger, Orianna
通讯作者：
Bretschger, Orianna
影响因子：
3.6
作者：
BONGAERTS, J;ZOSKE, S;UNDEN, G
通讯作者：
UNDEN, G
DOI：
10.1089/153623103322246566
发表时间：
2003-06-01
期刊：
OMICS A Journal of Integrative Biology
影响因子：
--
作者：
Daraselia, N.;Dernovoy, D.;Tatusova, T.
通讯作者：
Tatusova, T.
影响因子：
3.2
作者：
Hunt, Kristopher A.;Flynn, Jeffrey M.;Gralnick, Jeffrey A.
通讯作者：
Gralnick, Jeffrey A.