Ontogeny of circadian organization in the rat.
Ontogeny of circadian organization in the rat.
复制标题
大鼠昼夜节律组织的个体发育。
DOI：
10.1177/0748730408328438
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发表时间：
2009-02
影响因子：
3.5
通讯作者：
Menaker, Michael
中科院分区：
文献类型：
--
作者：
Yamazaki, Shin;Yoshikawa, Tomoko;Biscoe, Elizabeth W.;Numano, Rika;Gallaspy, Lauren M.;Soulsby, Stacy;Papadimas, Evagelia;Pezuk, Pinar;Doyle, Susan E.;Tei, Hajime;Sakaki, Yoshiyuki;Block, Gene D.;Menaker, Michael
关键词：
哺乳动物的昼夜节律系统由大脑中的主起搏器协调，但许多外周组织也包含独立或准独立的昼夜节律振荡器。在这些结构中，时钟的适应意义在很大程度上取决于组成振荡器与其微观和宏观环境之间的相位关系。要检查出生后的发展，这是依赖于内源性程序和母亲/环境的影响，和昼夜节律振荡器的阶段之间的关系，我们评估了松果体，肝脏，肺，肾上腺，甲状腺组织培养的周期1-荧光素酶（Per 1-luc）大鼠幼崽的各种出生后年龄的昼夜节律阶段。肝脏，甲状腺和松果体的节奏在出生时，但他们的Per 1-luc表达节奏的阶段转移显着的发展。为了确定每个组织中相移的时间是否可能是环境条件变化的结果，我们监测了幼崽及其母亲的行为。我们发现肝脏的昼夜节律阶段在出生后第22天左右从白天转移到夜晚，因为幼鼠在光照下喂养较少，而在黑暗中吃固体食物。此外，在哺乳期新生儿肝培养物中Per 1-luc表达的阶段可以通过实验从白天转移到晚上，只允许幼鼠在黑暗中接近母鼠。峰值Per 1-luc表达也从中午转移到深夜甲状腺文化在P20左右，同时在进食时间的转变。Per 1-luc在松果体中的表达时相从白天转移到晚上，与其交感神经支配在P5左右一致。Per 1-luc表达在肾上腺培养物中是有节律的，在整个发育过程中在熄灯时间附近达到峰值，然而，节律的幅度在P25时增加。肺培养物在P12前完全无菌，此时幼仔开始离巢。总之，我们的数据表明，产生昼夜节律振荡的分子机制在不同组织中以不同的速度成熟，并且至少一些外周器官的相位是可塑性的，并且可能随着器官功能在发育过程中的变化而变化。
The mammalian circadian system is orchestrated by a master pacemaker in the brain but many peripheral tissues also contain independent or quasi-independent circadian oscillators. The adaptive significance of clocks in these structures must lie, in large part, in the phase relationships between the constituent oscillators and their micro- and macro-environments. To examine the relationship between postnatal development, which is dependent on endogenous programs and maternal/environmental influences, and the phase of circadian oscillators, we assessed the circadian phase of pineal, liver, lung, adrenal, and thyroid tissues cultured from Period 1-luciferase (Per1-luc) rat pups of various postnatal ages. The liver, thyroid, and pineal were rhythmic at birth, but the phases of their Per1-luc expression rhythms shifted remarkably during development. To determine if the timing of the phase shift in each tissue could be the result of changing environmental conditions, we monitored the behavior of pups and their mothers. We found that the circadian phase of the liver shifted from the day to night around postnatal day (P) 22 as the pups nursed less during the light and instead ate solid food during the dark. Furthermore, the phase of Per1-luc expression in liver cultures from nursing neonates could be shifted experimentally from the day to the night by allowing pups access to the dam only during the dark. Peak Per1-luc expression also shifted from mid-day to early night in thyroid cultures at about P20, concurrent with the shift in eating times. The phase of Per1-luc expression in the pineal gland shifted from day to night coincident with its sympathetic innervation at around P5. Per1-luc expression was rhythmic in adrenal cultures and peaked around the time of lights-off throughout development, however, the amplitude of the rhythm increased at P25. Lung cultures were completely arrhythmic until P12 when the pups began to leave the nest. Taken together, our data suggest that the molecular machinery that generates circadian oscillations matures at different rates in different tissues and that the phase of at least some peripheral organs is malleable and may shift as the organ's function changes during development.
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DOI：
10.1152/ajpregu.00184.2006
发表时间：
2007-03-01
影响因子：
2.8
作者：
Sladek, Martin;Jindrakova, Zuzana;Sumova, Alena
通讯作者：
Sumova, Alena
DOI：
10.1152/ajpregu.00498.2007
发表时间：
2008-07-01
影响因子：
2.8
作者：
Vujovic, Nina;Davidson, Alec J.;Menaker, Michael
通讯作者：
Menaker, Michael
影响因子：
6.1
作者：
HAKANSON, R.;LOMBARD des GOUTTES, M.-N.;OWMAN, C.
通讯作者：
OWMAN, C.
影响因子：
3.4
作者：
Ohta, H;Honma, S;Honma, K
通讯作者：
Honma, K
影响因子：
3.7
作者：
Ohta, Hidenobu;Xu, Shanhai;Okamura, Kunihiro
通讯作者：
Okamura, Kunihiro