Deep learning-based transformation of H&E stained tissues into special stains.
Deep learning-based transformation of H&E stained tissues into special stains.
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DOI：
10.1038/s41467-021-25221-2
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发表时间：
2021-08-12
影响因子：
16.6
通讯作者：
Ozcan A
中科院分区：
文献类型：
--
作者：
de Haan K;Zhang Y;Zuckerman JE;Liu T;Sisk AE;Diaz MFP;Jen KY;Nobori A;Liou S;Zhang S;Riahi R;Rivenson Y;Wallace WD;Ozcan A
病理是通过肉眼检查组织化学染色的组织切片。虽然苏木精和伊红（H&E）染色是最常用的，特殊的染色可以提供额外的对比不同的组织成分。在这里，我们展示了使用肾针核心活检组织切片从H&E到特殊染色（马森三色，周期性酸-希夫和琼斯银染色）的基于监督学习的计算染色转换的实用价值。基于三位肾脏病理学家的评估，以及第四位病理学家的判断，我们发现从现有的H&E图像中生成虚拟特殊染色可以提高对58个不同受试者的几种非肿瘤性肾脏疾病的诊断（P = 0.0095）。第二项研究发现，计算生成的特殊染色的质量在统计上与组织化学染色的质量相当。当需要额外的特殊染色剂时，这种染色到染色的转化框架可以改善初步诊断，也可以显着节省时间和成本。在活组织检查中进行多次组织学染色既昂贵又耗时。在这里，作者提出了一种将H&E染色的组织数字化转化为特殊染色的方法（例如，PAS，马森三色和琼斯银染色），并证明它比仅使用H&E能提高诊断。
Pathology is practiced by visual inspection of histochemically stained tissue slides. While the hematoxylin and eosin (H&E) stain is most commonly used, special stains can provide additional contrast to different tissue components. Here, we demonstrate the utility of supervised learning-based computational stain transformation from H&E to special stains (Masson’s Trichrome, periodic acid-Schiff and Jones silver stain) using kidney needle core biopsy tissue sections. Based on the evaluation by three renal pathologists, followed by adjudication by a fourth pathologist, we show that the generation of virtual special stains from existing H&E images improves the diagnosis of several non-neoplastic kidney diseases, sampled from 58 unique subjects (P = 0.0095). A second study found that the quality of the computationally generated special stains was statistically equivalent to those which were histochemically stained. This stain-to-stain transformation framework can improve preliminary diagnoses when additional special stains are needed, also providing significant savings in time and cost. Performing multiple histological stains on a biopsy can be costly and time consuming. Here the authors present a method for the digital transformation of H&E stained tissue into special stains (e.g., PAS, Masson’s Trichrome and Jones silver stain), and demonstrate that it improves diagnoses over the use of H&E only.
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DOI：
10.5539/gjhs.v8n3p72
发表时间：
2015-06-25
期刊：
Global journal of health science
影响因子：
--
作者：
Alturkistani HA;Tashkandi FM;Mohammedsaleh ZM
通讯作者：
Mohammedsaleh ZM
DOI：
10.1109/jbhi.2020.2975151
发表时间：
2021-02-01
影响因子：
7.7
作者：
Lahiani, Amal;Klaman, Irina;Klaiman, Eldad
通讯作者：
Klaiman, Eldad
影响因子：
4.6
作者：
Sandfort, Veit;Yan, Ke;Summers, Ronald M.
通讯作者：
Summers, Ronald M.
影响因子：
28.1
作者：
Glaser, Adam K.;Reder, Nicholas P.;Liu, Jonathan T. C.
通讯作者：
Liu, Jonathan T. C.
影响因子：
4.6
作者：
Pantanowitz L;Sinard JH;Henricks WH;Fatheree LA;Carter AB;Contis L;Beckwith BA;Evans AJ;Lal A;Parwani AV;College of American Pathologists Pathology and Laboratory Quality Center
通讯作者：
College of American Pathologists Pathology and Laboratory Quality Center