Fiber enhancement and 3D orientation analysis in label-free two-photon fluorescence microscopy.
Fiber enhancement and 3D orientation analysis in label-free two-photon fluorescence microscopy.
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DOI：
10.1038/s41598-023-30953-w
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发表时间：
2023-03-13
影响因子：
4.6
通讯作者：
Mazzamuto, Giacomo
中科院分区：
文献类型：
--
作者：
Sorelli, Michele;Costantini, Irene;Bocchi, Leonardo;Axer, Markus;Pavone, Francesco Saverio;Mazzamuto, Giacomo
荧光显微镜可用于评估大脑的纤维结构，具有无与伦比的空间分辨率，并结合不同的组织制备和染色方案。与最先进的基于偏振测量的神经成像模式不同，荧光显微镜体积图像的纤维束方向的量化需要应用特定的图像处理技术，例如傅里叶或结构张量分析。然而，这些可能会导致不可靠的结果，因为它们不会将有髓纤维与周围组织隔离。在这项工作中，我们描述了一种新颖的图像处理管道，该管道能够利用 Frangi 滤波器的 3D 实现提供的不同直径的管状结构的选择性多尺度增强，从灰质和白质区域计算精确的 3D 纤维取向图。开发的软件工具可以有效地生成任意空间尺度的方向分布函数图，这可以支持现代扩散加权磁共振成像纤维束成像的组织学验证。尽管在这里对双光子扫描荧光显微镜图像进行了测试，这些图像是从用无标记技术处理的组织样本中获取的，增强了有髓纤维的自发荧光，但所提出的流程被开发用于所有类型的 3D 荧光图像和纤维染色。
Fluorescence microscopy can be exploited for evaluating the brain’s fiber architecture with unsurpassed spatial resolution in combination with different tissue preparation and staining protocols. Differently from state-of-the-art polarimetry-based neuroimaging modalities, the quantification of fiber tract orientations from fluorescence microscopy volume images entails the application of specific image processing techniques, such as Fourier or structure tensor analysis. These, however, may lead to unreliable outcomes as they do not isolate myelinated fibers from the surrounding tissue. In this work, we describe a novel image processing pipeline that enables the computation of accurate 3D fiber orientation maps from both grey and white matter regions, exploiting the selective multiscale enhancement of tubular structures of varying diameters provided by a 3D implementation of the Frangi filter. The developed software tool can efficiently generate orientation distribution function maps at arbitrary spatial scales which may support the histological validation of modern diffusion-weighted magnetic resonance imaging tractography. Despite being tested here on two-photon scanning fluorescence microscopy images, acquired from tissue samples treated with a label-free technique enhancing the autofluorescence of myelinated fibers, the presented pipeline was developed to be employed on all types of 3D fluorescence images and fiber staining.
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通过三维偏振光成像，人脑中的高分辨率纤维道重建。
影响因子：
3.5
作者：
Axer M;Grässel D;Kleiner M;Dammers J;Dickscheid T;Reckfort J;Hütz T;Eiben B;Pietrzyk U;Zilles K;Amunts K
通讯作者：
Amunts K
影响因子：
4
作者：
Giardini F;Lazzeri E;Vitale G;Ferrantini C;Costantini I;Pavone FS;Poggesi C;Bocchi L;Sacconi L
通讯作者：
Sacconi L
影响因子：
48
作者：
Chung, Kwanghun;Deisseroth, Karl
通讯作者：
Deisseroth, Karl
影响因子：
5.7
作者：
Khan AR;Cornea A;Leigland LA;Kohama SG;Jespersen SN;Kroenke CD
通讯作者：
Kroenke CD
影响因子：
4.6
作者：
Costantini I;Baria E;Sorelli M;Matuschke F;Giardini F;Menzel M;Mazzamuto G;Silvestri L;Cicchi R;Amunts K;Axer M;Pavone FS
通讯作者：
Pavone FS