Transcriptomic dissection of tongue squamous cell carcinoma.
Transcriptomic dissection of tongue squamous cell carcinoma.
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DOI：
10.1186/1471-2164-9-69
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发表时间：
2008-02-06
期刊：
影响因子：
4.4
通讯作者：
Zhou X
中科院分区：
文献类型：
--
作者：
Ye H;Yu T;Temam S;Ziober BL;Wang J;Schwartz JL;Mao L;Wong DT;Zhou X
头颈/口腔鳞状细胞癌 (HNOSCC) 是一组多样化的癌症，由许多不同的解剖部位发展而来，并与不同的危险因素和遗传特征相关。口腔舌鳞状细胞癌（OTSCC）是最常见的 HNOSCC 类型之一。在局部侵袭和扩散方面，它比其他形式的 HNOSCC 更具侵袭性。在这项研究中，我们的目标是鉴定与 OTSCC 相关的特定转录组特征。获得了 53 个原发性 OTSCC 和 22 个匹配的正常组织的全基因组转录组图谱。鉴定出在 OTSCC 和正常人之间表达具有统计学显着差异的基因。其中包括上调基因（MMP1、MMP10、MMP3、MMP12、PTHLH、INHBA、LAMC2、IL8、KRT17、COL1A2、IFI6、ISG15、PLAU、GREM1、MMP9、IFI44、CXCL1）和下调基因（KRT4、MAL、CRNN、SCEL、CRISP3、SPINK5、 CLCA4、ADH1B、P11、TGM3、RHCG、PPP1R3C、CEACAM7、HPGD、CFD、ABCA8、CLU、CYP3A5)。通过实时定量RT-PCR和免疫组化进一步验证IL8和MMP9的表达差异。基因本体分析表明 OTSCC 中存在许多改变的生物过程，包括磷酸盐转运、胶原蛋白分解代谢、I-kappaB 激酶/NF-kappaB 信号级联、细胞外基质组织和生物发生、趋化性以及超氧化物释放、过氧化氢代谢、细胞对过氧化氢的反应的抑制的增强， OTSCC 中的角化和角化细胞分化。总之，我们的研究提供了 OTSCC 的转录组特征，可能导致诊断或筛选工具，并为 OTSCC 的这些特定候选基因的进一步功能验证提供基础。
The head and neck/oral squamous cell carcinoma (HNOSCC) is a diverse group of cancers, which develop from many different anatomic sites and are associated with different risk factors and genetic characteristics. The oral tongue squamous cell carcinoma (OTSCC) is one of the most common types of HNOSCC. It is significantly more aggressive than other forms of HNOSCC, in terms of local invasion and spread. In this study, we aim to identify specific transcriptomic signatures that associated with OTSCC. Genome-wide transcriptomic profiles were obtained for 53 primary OTSCCs and 22 matching normal tissues. Genes that exhibit statistically significant differences in expression between OTSCCs and normal were identified. These include up-regulated genes (MMP1, MMP10, MMP3, MMP12, PTHLH, INHBA, LAMC2, IL8, KRT17, COL1A2, IFI6, ISG15, PLAU, GREM1, MMP9, IFI44, CXCL1), and down-regulated genes (KRT4, MAL, CRNN, SCEL, CRISP3, SPINK5, CLCA4, ADH1B, P11, TGM3, RHCG, PPP1R3C, CEACAM7, HPGD, CFD, ABCA8, CLU, CYP3A5). The expressional difference of IL8 and MMP9 were further validated by real-time quantitative RT-PCR and immunohistochemistry. The Gene Ontology analysis suggested a number of altered biological processes in OTSCCs, including enhancements in phosphate transport, collagen catabolism, I-kappaB kinase/NF-kappaB signaling cascade, extracellular matrix organization and biogenesis, chemotaxis, as well as suppressions of superoxide release, hydrogen peroxide metabolism, cellular response to hydrogen peroxide, keratinization, and keratinocyte differentiation in OTSCCs. In summary, our study provided a transcriptomic signature for OTSCC that may lead to a diagnosis or screen tool and provide the foundation for further functional validation of these specific candidate genes for OTSCC.
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影响因子：
6.4
作者：
Annertz, K;Anderson, H;Wennerberg, J
通讯作者：
Wennerberg, J
影响因子：
12.3
作者：
Gentleman RC;Carey VJ;Bates DM;Bolstad B;Dettling M;Dudoit S;Ellis B;Gautier L;Ge Y;Gentry J;Hornik K;Hothorn T;Huber W;Iacus S;Irizarry R;Leisch F;Li C;Maechler M;Rossini AJ;Sawitzki G;Smith C;Smyth G;Tierney L;Yang JY;Zhang J
通讯作者：
Zhang J
影响因子：
4.8
作者：
Mackenzie, J;Ah-See, K;Macfarlane, GJ
通讯作者：
Macfarlane, GJ
影响因子：
6.3
作者：
Izutani, R;Asano, S;Ohyanagi, H
通讯作者：
Ohyanagi, H
影响因子：
8.8
作者：
Janssen AM;Bosman CB;Sier CF;Griffioen G;Kubben FJ;Lamers CB;van Krieken JH;van de Velde CJ;Verspaget HW
通讯作者：
Verspaget HW