Epigenome-wide association study of lung function in Latino children and youth with asthma.
Epigenome-wide association study of lung function in Latino children and youth with asthma.
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哮喘的拉丁裔儿童和青少年的肺功能全基因组协会研究。
DOI：
10.1186/s13148-022-01227-5
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发表时间：
2022-01-15
影响因子：
5.7
通讯作者：
Pino-Yanes M
中科院分区：
文献类型：
--
作者：
Herrera-Luis E;Li A;Mak ACY;Perez-Garcia J;Elhawary JR;Oh SS;Hu D;Eng C;Keys KL;Huntsman S;Beckman KB;Borrell LN;Rodriguez-Santana J;Burchard EG;Pino-Yanes M
DNA甲基化研究将不同CpG位点或基因组区域的甲基化水平与肺功能联系起来。此外，遗传祖先与拉丁美洲人的肺功能有关。然而，没有在这一人群中进行肺功能的全表观基因组关联研究（EWAS）。在这里，我们的目的是确定拉丁美洲儿童哮喘中与肺功能相关的DNA甲基化模式。我们对250名患有哮喘的波多黎各人和148名墨西哥裔美国儿童和青年进行了全血EWAS。在波多黎各人和墨西哥裔美国人的组合分析中，共有5个CpGs超过了p = 1.17 × 10−7的全基因组显著性阈值：cg06035600 （MAP3K6, p = 6.13 × 10−8）与支气管扩张前的Tiffeneau-Pinelli指数显著相关，cg00914963 （TBC1D16, p = 1.04 × 10−7）、cg16405908 （MRGPRE, p = 2.05 × 10−8）和cg07428101 （MUC2, p = 5.02 × 10−9）与支气管扩张后的强制肺活量（FVC）相关，cg20515679 （KCNJ6）与支气管扩张后的Tiffeneau-Pinelli指数相关（p = 1.13 × 10−8）。然而，这些标记在欧洲人的公开数据中没有显示出显著的相关性（p > 0.05）。一项甲基化数量性状位点分析显示，这些CpG位点的甲基化水平受到拉丁美洲人和基于生物银行的整合组学研究（BIOS）联盟的遗传变异的调节。此外，波多黎各人和墨西哥裔美国人的REXOC和AURKC的两个差异甲基化区域与支气管扩张剂前Tiffeneau-Pinelli指数相关（调整p < 0.05）。此外，我们在非拉丁裔人群中复制了一些先前与肺功能相关的差异甲基化信号。我们重复了之前全血中表观遗传标记与肺功能的关联，并确定了拉丁裔亚群中共享的新的人群特异性关联。在线版本包含补充材料，可在10.1186/s13148-022-01227-5获得。
DNA methylation studies have associated methylation levels at different CpG sites or genomic regions with lung function. Moreover, genetic ancestry has been associated with lung function in Latinos. However, no epigenome-wide association study (EWAS) of lung function has been performed in this population. Here, we aimed to identify DNA methylation patterns associated with lung function in pediatric asthma among Latinos. We conducted an EWAS in whole blood from 250 Puerto Rican and 148 Mexican American children and young adults with asthma. A total of five CpGs exceeded the genome-wide significance threshold of p = 1.17 × 10−7 in the combined analyses from Puerto Ricans and Mexican Americans: cg06035600 (MAP3K6, p = 6.13 × 10−8) showed significant association with pre-bronchodilator Tiffeneau–Pinelli index, the probes cg00914963 (TBC1D16, p = 1.04 × 10−7), cg16405908 (MRGPRE, p = 2.05 × 10−8), and cg07428101 (MUC2, p = 5.02 × 10−9) were associated with post-bronchodilator forced vital capacity (FVC), and cg20515679 (KCNJ6) with post-bronchodilator Tiffeneau–Pinelli index (p = 1.13 × 10−8). However, these markers did not show significant associations in publicly available data from Europeans (p > 0.05). A methylation quantitative trait loci analysis revealed that methylation levels at these CpG sites were regulated by genetic variation in Latinos and the Biobank-based Integrative Omics Studies (BIOS) consortium. Additionally, two differentially methylated regions in REXOC and AURKC were associated with pre-bronchodilator Tiffeneau–Pinelli index (adjusted p < 0.05) in Puerto Ricans and Mexican Americans. Moreover, we replicated some of the previous differentially methylated signals associated with lung function in non-Latino populations. We replicated previous associations of epigenetic markers with lung function in whole blood and identified novel population-specific associations shared among Latino subgroups. The online version contains supplementary material available at 10.1186/s13148-022-01227-5.
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影响因子：
3.5
作者：
Nedeljkovic I;Lahousse L;Carnero-Montoro E;Faiz A;Vonk JM;de Jong K;van der Plaat DA;van Diemen CC;van den Berge M;Obeidat M;Bossé Y;Nickle DC;Consortium BIOS;Uitterlinden AG;van Meurs JBJ;Stricker BHC;Brusselle GG;Postma DS;Boezen HM;van Duijn CM;Amin N
通讯作者：
Amin N
影响因子：
3.8
作者：
Lee MK;Hong Y;Kim SY;Kim WJ;London SJ
通讯作者：
London SJ
影响因子：
5.8
作者：
de Vries M;van der Plaat DA;Nedeljkovic I;Verkaik-Schakel RN;Kooistra W;Amin N;van Duijn CM;Brandsma CA;van Diemen CC;Vonk JM;Marike Boezen H
通讯作者：
Marike Boezen H
影响因子：
2.7
作者：
Fu, Xiang;Zhang, Fengling
通讯作者：
Zhang, Fengling
影响因子：
4
作者：
Alrashoudi, Reem H.;Crane, Isabel J.;Alajez, Nehad M.
通讯作者：
Alajez, Nehad M.