Silencing of Cholinergic Basal Forebrain Neurons Using Archaerhodopsin Prolongs Slow-Wave Sleep in Mice.
Silencing of Cholinergic Basal Forebrain Neurons Using Archaerhodopsin Prolongs Slow-Wave Sleep in Mice.
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使用古视紫红质沉默胆碱能基底前脑神经元可延长小鼠的慢波睡眠
DOI：
10.1371/journal.pone.0130130
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Yu YQ
中科院分区：
文献类型：
--
作者：
Shi YF;Han Y;Su YT;Yang JH;Yu YQ
基底前脑（BF）在皮质激活中起着至关重要的作用。我们以前的研究表明，单独激活胆碱能BF神经元就足以抑制慢波睡眠（SWS），促进觉醒和快速眼动（REM）睡眠。然而，沉默胆碱能BF神经元在睡眠-觉醒周期中的确切作用仍不清楚。我们用黄光抑制古视紫红质（Arch）基因靶向的胆碱能BF神经元，以阐明胆碱能BF神经元在睡眠-觉醒周期中的作用。用古视紫红质基因靶向的胆碱能BF神经元的双侧失活延长了小鼠SWS并降低了从SWS中醒来的概率。然而，沉默这些神经元既不会改变觉醒或REM睡眠的持续时间，也不会改变从觉醒或REM睡眠过渡到其他睡眠-觉醒事件的可能性。此外，在非活动或活动期内沉默这些神经元6 h增加了SWS的持续时间，但牺牲了清醒的持续时间，以及增加了延长的SWS发作（120-240 s）的次数。觉醒的延迟增加补偿了失去的觉醒，因此24 h内SWS和觉醒的总持续时间保持稳定。我们的研究结果表明，这些神经元的主要作用是终止SWS，而清醒或REM睡眠可能是由胆碱能BF神经元与其他觉醒睡眠控制系统的合作。
The basal forebrain (BF) plays a crucial role in cortical activation. Our previous study showed that activation of cholinergic BF neurons alone is sufficient to suppress slow-wave sleep (SWS) and promote wakefulness and rapid-eye-movement (REM) sleep. However, the exact role of silencing cholinergic BF neurons in the sleep-wake cycle remains unclear. We inhibitied the cholinergic BF neurons genetically targeted with archaerhodopsin (Arch) with yellow light to clarify the role of cholinergic BF neurons in the sleep-wake cycle. Bilateral inactivation of cholinergic BF neurons genetically targeted with archaerhodopsin prolonged SWS and decreased the probability of awakening from SWS in mice. However, silencing these neurons changed neither the duration of wakefulness or REM sleep, nor the probability of transitions to other sleep-wake episodes from wakefulness or REM sleep. Furthermore, silencing these neurons for 6 h within the inactive or active period increased the duration of SWS at the expense of the duration of wakefulness, as well as increasing the number of prolonged SWS episodes (120-240 s). The lost wakefulness was compensated by a delayed increase of wakefulness, so the total duration of SWS and wakefulness during 24 h was kept stable. Our results indicate that the main effect of these neurons is to terminate SWS, whereas wakefulness or REM sleep may be determined by co-operation of the cholinergic BF neurons with other arousal-sleep control systems.
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影响因子：
25
作者：
通讯作者：
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影响因子：
3.4
作者：
Berntson, GG;Shafi, R;Sarter, M
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通讯作者：
de Lecea, Luis
影响因子：
64.8
作者：
Adamantidis, Antoine R.;Zhang, Feng;De Lecea, Luis
通讯作者：
De Lecea, Luis