Understanding the accessory viral proteins unique to the severe acute respiratory syndrome (SARS) coronavirus.
Understanding the accessory viral proteins unique to the severe acute respiratory syndrome (SARS) coronavirus.
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DOI：
10.1016/j.antiviral.2006.05.010
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发表时间：
2006-11
影响因子：
7.6
通讯作者：
Hong W
中科院分区：
文献类型：
--
作者：
Tan YJ;Lim SG;Hong W
2002年11月，一种名为严重急性呼吸综合征冠状病毒(SARS-CoV)的新型冠状病毒在广东感染了人类中国，随后该病毒在人与人之间的有效传播于2003年在全球30多个国家造成了深刻的干扰。最终，通过隔离控制了这一流行病，自2004年1月以来一直没有人感染病例的报告。然而，对SARS-CoV不同方面的研究并未减弱，因为尚不清楚这种病毒是否会重新出现，特别是因为它的来源和潜在的宿主(S)尚未解决。SARS-CoV基因组全长近30kb，包含14个潜在的开放阅读框架(ORF)。其中一些开放阅读框编码的基因与所有已知的冠状病毒中发现的蛋白同源，即复制酶基因(ORF1a和1b)和四种结构蛋白：核衣壳、棘突、膜和包膜，这些蛋白被认为是病毒复制所必需的。其余8个ORF编码辅助蛋白，长度从39到274个氨基酸不等，这是SARS冠状病毒所独有的。本文将从三个主要方面综述这些辅助病毒蛋白的研究进展：(1)感染者血清中抗辅助蛋白抗体的检测；(2)辅助蛋白的表达、加工和细胞定位；(3)辅助蛋白对细胞功能的影响。SARS-CoV辅助蛋白在其他冠状病毒中没有同源物，这些深入的分子和生化特征可能提供线索，解释为什么SARS-CoV会导致人类如此严重和快速的攻击，而感染人类的其他冠状病毒似乎更宽容。
A novel coronavirus, termed the severe acute respiratory syndrome coronavirus (SARS-CoV), infected humans in Guangdong, China, in November 2002 and the subsequent efficient human-to-human transmissions of this virus caused profound disturbances in over 30 countries worldwide in 2003. Eventually, this epidemic was controlled by isolation and there has been no human infection reported since January 2004. However, research on different aspects of the SARS-CoV is not waning, as it is not known if this virus will re-emerge, especially since its origins and potential reservoir(s) are unresolved. The SARS-CoV genome is nearly 30 kb in length and contains 14 potential open reading frames (ORFs). Some of these ORFs encode for genes that are homologous to proteins found in all known coronaviruses, namely the replicase genes (ORFs 1a and 1b) and the four structural proteins: nucleocapsid, spike, membrane and envelope, and these proteins are expected to be essential for the replication of the virus. The remaining eight ORFs encodes for accessory proteins, varying in length from 39 to 274 amino acids, which are unique to SARS-CoV. This review will summarize the expeditious research on these accessory viral proteins in three major areas: (i) the detection of antibodies against accessory proteins in the serum of infected patients, (ii) the expression, processing and cellular localization of the accessory proteins, and (iii) the effects of the accessory proteins on cellular functions. These in-depth molecular and biochemical characterizations of the SARS-CoV accessory proteins, which have no homologues in other coronaviruses, may offer clues as to why the SARS-CoV causes such a severe and rapid attack in humans, while other coronaviruses that infect humans seem to be more forgiving.
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DOI：
10.1038/modpathol.3800439
发表时间：
2005-11
期刊：
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc
影响因子：
--
作者：
Chan WS;Wu C;Chow SC;Cheung T;To KF;Leung WK;Chan PK;Lee KC;Ng HK;Au DM;Lo AW
通讯作者：
Lo AW
影响因子：
9.4
作者：
Che, XY;Qiu, LW;Yuen, KY
通讯作者：
Yuen, KY
DOI：
10.1128/cdli.11.4.699-703.2004
发表时间：
2004-07-01
期刊：
CLINICAL AND DIAGNOSTIC LABORATORY IMMUNOLOGY
影响因子：
--
作者：
Guan, M;Chan, KH;Wong, CJ
通讯作者：
Wong, CJ
影响因子：
5.4
作者：
Callahan, MA;Handley, MA;Panganiban, AT
通讯作者：
Panganiban, AT
影响因子：
3.3
作者：
Franks TJ;Chong PY;Chui P;Galvin JR;Lourens RM;Reid AH;Selbs E;McEvoy CP;Hayden CD;Fukuoka J;Taubenberger JK;Travis WD
通讯作者：
Travis WD