Characterization and Mapping of Leaf Rust and Stripe Rust Resistance Loci in Hexaploid Wheat Lines UC1110 and PI610750 under Mexican Environments.
Characterization and Mapping of Leaf Rust and Stripe Rust Resistance Loci in Hexaploid Wheat Lines UC1110 and PI610750 under Mexican Environments.
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在墨西哥环境下，在六倍层小麦线UC1110和PI610750中叶片锈和条纹抗锈蚀位点的表征和映射。
DOI：
10.3389/fpls.2017.01450
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发表时间：
2017
影响因子：
5.6
通讯作者：
Singh RP
中科院分区：
文献类型：
--
作者：
Lan C;Hale IL;Herrera-Foessel SA;Basnet BR;Randhawa MS;Huerta-Espino J;Dubcovsky J;Singh RP
种植抗病小麦品种是将由全球重要的小麦叶锈病（LR）和条锈病（YR）所导致的产量损失程度降至最低的关键方法。在本研究中，对186个F8重组自交系（RILs）群体在多个季节的幼苗期和成株期进行了叶锈病和条锈病反应的表型分析，该群体来自一个人工合成小麦衍生品种（PI610750）和一个适应性良好的普通小麦品系（品种“UC1110”）的杂交。利用由单序列重复和多样性芯片技术标记组成的遗传连锁图谱，结合包容性复合区间作图分析，我们检测到一个新的叶锈病成株抗性（APR）位点QLr.cim - 2DS，由UC1110提供。还定位到了一个对两种锈病都共定位的抗性位点QLr.cim - 3DC/QYr.cim - 3DC，以及已知的幼苗抗性基因Lr26。QLr.cim - 2DS和QLr.cim - 3DC在成株期对叶锈病抗性表现出边缘显著的相互作用。此外，两个先前报道的条锈病成株抗性位点QYr.ucw - 3BS和Yr48在墨西哥和美国的锈病环境中表现稳定，并且在田间表现出高度显著的相互作用。还观察到Yr48对墨西哥条锈病菌株具有中等的幼苗抗性，因此建议将其重新归类为全生育期抗性基因。我们还分别鉴定出5个和2个拥有所有已检测到的条锈病和叶锈病抗性位点的重组自交系。利用此处报道的紧密连锁的分子标记，这些重组自交系可在小麦育种项目中用作对两种锈病具有多个抗性位点的供体。
Growing resistant wheat varieties is a key method of minimizing the extent of yield losses caused by the globally important wheat leaf rust (LR) and stripe rust (YR) diseases. In this study, a population of 186 F8 recombinant inbred lines (RILs) derived from a cross between a synthetic wheat derivative (PI610750) and an adapted common wheat line (cv. “UC1110”) were phenotyped for LR and YR response at both seedling and adult plant stages over multiple seasons. Using a genetic linkage map consisting of single sequence repeats and diversity arrays technology markers, in combination with inclusive composite interval mapping analysis, we detected a new LR adult plant resistance (APR) locus, QLr.cim-2DS, contributed by UC1110. One co-located resistance locus to both rusts, QLr.cim-3DC/QYr.cim-3DC, and the known seedling resistance gene Lr26 were also mapped. QLr.cim-2DS and QLr.cim-3DC showed a marginally significant interaction for LR resistance in the adult plant stage. In addition, two previously reported YR APR loci, QYr.ucw-3BS and Yr48, were found to exhibit stable performances in rust environments in both Mexico and the United States and showed a highly significant interaction in the field. Yr48 was also observed to confer intermediate seedling resistance against Mexican YR races, thus suggesting it should be re-classified as an all-stage resistance gene. We also identified 5 and 2 RILs that possessed all detected YR and LR resistance loci, respectively. With the closely linked molecular markers reported here, these RILs could be used as donors for multiple resistance loci to both rusts in wheat breeding programs.
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影响因子：
2
作者：
Landjeva, S;Korzun, V;Ganeva, G
通讯作者：
Ganeva, G
影响因子：
56.9
作者：
Krattinger, Simon G.;Lagudah, Evans S.;Keller, Beat
通讯作者：
Keller, Beat
影响因子：
3.2
作者：
BJARKO M E;LINE R F
通讯作者：
LINE R F
影响因子：
5.4
作者：
Hiebert, Colin W.;Thomas, Julian B.;Fox, Stephen L.
通讯作者：
Fox, Stephen L.
影响因子：
1.9
作者：
Lowe, Iago;Cantu, Dario;Dubcovsky, Jorge
通讯作者：
Dubcovsky, Jorge