Arabidopsis thaliana PRR7 Provides Circadian Input to the CCA1 Promoter in Shoots but not Roots.
Arabidopsis thaliana PRR7 Provides Circadian Input to the CCA1 Promoter in Shoots but not Roots.
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DOI：
10.3389/fpls.2021.750367
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发表时间：
2021
影响因子：
5.6
通讯作者：
Laird J
中科院分区：
文献类型：
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作者：
Nimmo HG;Laird J
植物生物钟的核心涉及多个互锁的基因表达环和翻译后控制以及光和新陈代谢的输入。交互的复杂性使得很少有特定功能可以归因于单个组件。在之前的工作中，我们报道了拟南芥芽和根中时钟运行的差异，包括关键时钟组件突变的影响。在这里，我们使用荧光素酶成像来研究表达 CCA1::LUC 和 GI::LUC 标记的 prr7 突变体。在表达 CCA1::LUC 的成熟芽中，PRR7 的缺失从根本上改变了光：暗循环中的行为，并导致恒定光下节律性的丧失，但对根的影响很小。相比之下，在表达 GI::LUC 的成熟植物中，PRR7 的缺失对光：暗循环几乎没有影响，但在恒定光照下，芽的昼夜节律会增加，根的昼夜节律会减少。我们得出的结论是，芽中 CCA1 启动子的大部分或全部昼夜节律输入是由 PRR7 介导的，并且 PRR7 的丢失具有器官特异性影响。结果强调了芽和根时钟运行的差异，以及研究光：暗周期和恒定光下时钟突变体的重要性。
The core of the plant circadian clock involves multiple interlocking gene expression loops and post-translational controls along with inputs from light and metabolism. The complexity of the interactions is such that few specific functions can be ascribed to single components. In previous work, we reported differences in the operation of the clocks in Arabidopsis shoots and roots, including the effects of mutations of key clock components. Here, we have used luciferase imaging to study prr7 mutants expressing CCA1::LUC and GI::LUC markers. In mature shoots expressing CCA1::LUC, loss of PRR7 radically altered behaviour in light:dark cycles and caused loss of rhythmicity in constant light but had little effect on roots. In contrast, in mature plants expressing GI::LUC, loss of PRR7 had little effect in light:dark cycles but in constant light increased the circadian period in shoots and reduced it in roots. We conclude that most or all of the circadian input to the CCA1 promoter in shoots is mediated by PRR7 and that loss of PRR7 has organ-specific effects. The results emphasise the differences in operation of the shoot and root clocks, and the importance of studying clock mutants in both light:dark cycles and constant light.
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