Integration of deep transcriptome and proteome analyses of salicylic acid regulation high temperature stress in Ulva prolifera.
Integration of deep transcriptome and proteome analyses of salicylic acid regulation high temperature stress in Ulva prolifera.
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DOI：
10.1038/s41598-017-11449-w
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发表时间：
2017-09-08
影响因子：
4.6
通讯作者：
Miao Z
中科院分区：
文献类型：
--
作者：
Fan M;Sun X;Xu N;Liao Z;Li Y;Wang J;Fan Y;Cui D;Li P;Miao Z
研究水杨酸调节美国耐热性时转录本和相对蛋白水平的变化。增殖，互补转录组和蛋白质组分析与U。在35 °C（UpHT）和在高温下添加SA（UpSHT）下生长的增殖藻。在mRNA水平上，通过UpSHT和UpHT的比较，共获得12，296个差异表达基因（DEG）。iTRAQ标记蛋白质组分析显示，共鉴定并可靠定量了4，449种蛋白质。在mRNA水平上，与抗氧化活性相关的上调基因有硫氧还蛋白、过氧化物酶、FeSOD、谷胱甘肽过氧化物酶、过氧化氢酶和MnSOD。下调的基因有抗坏血酸过氧化物酶、谷胱甘肽S-转移酶、过氧化氢酶和MnSOD。此外，参与植物信号转导途径的DEG（如生长素反应因子、BRI 1和JAZ）表达下调。在蛋白质水平上，表达上调的碳固定相关蛋白和表达下调的蛋白主要是聚泛素和抗坏血酸过氧化物酶。Ca ~（2+）结合蛋白、热激蛋白和光合相关蛋白EDS 1的表达在mRNA和蛋白水平上也受到显著调控。结果表明，水杨酸通过上调抗氧化相关蛋白的表达，增强JA信号通路，改变Ca 2+结合蛋白、光合相关蛋白，提高抗氧化酶活性，改变光合指数，减轻高温胁迫的影响。
To investigate changes in transcript and relative protein levels in response to salicylic acid regulation of the thermotolerance in U. prolifera, complementary transcriptome and proteome analyses were performed with U. prolifera grown at 35 °C (UpHT) and with the addition of SA at high temperature (UpSHT). At mRNA level,12,296 differentially expressed genes (DEGs) were obtained from the comparison of UpSHT with UpHT. iTRAQ-labeling proteome analysis showed that a total of 4,449 proteins were identified and reliably quantified. At mRNA level, the up-regulated genes involved in antioxidant activity were thioredoxin,peroxiredoxin,FeSOD, glutathione peroxidase, partion catalase and MnSOD. The down-regulated genes were ascorbate peroxidase, glutathione S-transferase, catalase and MnSOD. In addition, the DEGs involved in plant signal transduction pathway (such as auxin response factors, BRI1 and JAZ) were down-regulated. At protein level, the up-regulated proteins involved in carbon fixation and the down-regulated protein mainly were polyubiquitin, ascorbate peroxidase. The expression of Ca2+-binding protein, heat shock protein and photosynthesis-related proteins, EDS1 were also significantly regulated both at mRNA and protein level. The results indicated that SA alleviated the high-temperature stimulus through partion antioxidant related proteins up-regulated, JA signal pathway enchanced, Ca2+-binding proteins, photosynthesis-related proteins significantly changed, antioxidant enzyme activities increased and photosynthesis index changed.
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影响因子：
6.9
作者：
Bastías A;Yañez M;Osorio S;Arbona V;Gómez-Cadenas A;Fernie AR;Casaretto JA
通讯作者：
Casaretto JA
影响因子：
2.5
作者：
Li, Youxun;Zhang, Xiaowen;Ye, Naihao
通讯作者：
Ye, Naihao
细胞分裂素通过促进拟南芥中脱落酸不敏感5蛋白的降解来拮抗脱落酸介导的子叶绿化抑制
影响因子：
7.4
作者：
Guan, Chunmei;Wang, Xingchun;Zuo, Jianru
通讯作者：
Zuo, Jianru
影响因子：
14.9
作者：
Franceschini A;Szklarczyk D;Frankild S;Kuhn M;Simonovic M;Roth A;Lin J;Minguez P;Bork P;von Mering C;Jensen LJ
通讯作者：
Jensen LJ
影响因子：
7
作者：
Chen, Zhen;Wen, Bo;Liu, Siqi
通讯作者：
Liu, Siqi