The transcription factor AREB1 regulates primary metabolic pathways in tomato fruits.
The transcription factor AREB1 regulates primary metabolic pathways in tomato fruits.
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DOI：
10.1093/jxb/eru114
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发表时间：
2014-06
影响因子：
6.9
通讯作者：
Casaretto JA
中科院分区：
文献类型：
--
作者：
Bastías A;Yañez M;Osorio S;Arbona V;Gómez-Cadenas A;Fernie AR;Casaretto JA
描述了具有不同SlAREB 1表达水平的番茄果实中的代谢物含量和初级代谢基因的表达。参与此转录因子和脱落酸信号在果实成熟过程中的代谢编程的建议。番茄果实的发育受植物激素的作用和严格的遗传控制。最近的研究表明，脱落酸（阿坝）信号可能会影响果实成熟的不同方面。以前，它被证明，SLAREB 1，ABA调节的转录因子参与胁迫诱导的反应，在番茄种子和果实组织中表达。在这里，使用转基因番茄品系研究了SlAREB 1在调节与主要代谢途径相关的基因表达和影响果实代谢谱中的作用。采用气相色谱-飞行时间质谱（GC-TOF-MS）和非靶向液相色谱-质谱（LC-MS）对果实发育三个阶段的果皮组织进行代谢物分析。数据的主成分分析可以区分非转基因水果的代谢产物谱与过表达和下调SlAREB 1的水果的代谢产物谱。过表达SlAREB 1导致未成熟绿色、成熟绿色和红色成熟果实中有机酸、己糖、己糖磷酸和氨基酸含量增加，这些修饰与参与初级碳水化合物和氨基酸代谢的酶编码基因的上调相关。非靶向LC-MS分析表明，转基因品系中次级代谢产物的组成也受到影响。此外，基因表达数据显示，与野生型和反义系相比，一些与果实成熟相关的基因在SlAREB 1过表达系中也上调。两者合计，结果表明，SlAREB 1参与调节的代谢程序，发生在果实成熟过程中，这可能解释的阿坝在番茄果实发育中的作用的一部分。
Metabolite contents and expression of genes of primary metabolism are described in tomato fruits with different SlAREB1 expression levels. Participation of this transcription factor and abscisic acid signalling in metabolic programming during fruit ripening is suggested. Tomato fruit development is regulated both by the action of plant hormones and by tight genetic control. Recent studies suggest that abscisic acid (ABA) signalling may affect different aspects of fruit maturation. Previously, it was shown that SlAREB1, an ABA-regulated transcription factor involved in stress-induced responses, is expressed in seeds and in fruit tissues in tomato. Here, the role of SlAREB1 in regulating the expression of genes relevant for primary metabolic pathways and affecting the metabolic profile of the fruit was investigated using transgenic tomato lines. Metabolite profiling using gas chromatography–time of flight mass spectrometry (GC-TOF-MS) and non-targeted liquid chromatography–mass spectrometry (LC-MS) was performed on pericarp tissue from fruits harvested at three stages of fruit development. Principal component analysis of the data could distinguish the metabolite profiles of non-transgenic fruits from those that overexpress and down-regulate SlAREB1. Overexpression of SlAREB1 resulted in increased content of organic acids, hexoses, hexose-phosphates, and amino acids in immature green, mature green, and red ripe fruits, and these modifications correlated with the up-regulation of enzyme-encoding genes involved in primary carbohydrate and amino acid metabolism. A non-targeted LC-MS analysis indicated that the composition of secondary metabolites is also affected in transgenic lines. In addition, gene expression data revealed that some genes associated with fruit ripening are also up-regulated in SlAREB1-overexpressing lines compared with wild-type and antisense lines. Taken together, the results suggest that SlAREB1 participates in the regulation of the metabolic programming that takes place during fruit ripening and that may explain part of the role of ABA in fruit development in tomato.
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