Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
Comparison of bone marrow and adipose tissue-derived canine mesenchymal stem cells.
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DOI：
10.1186/1746-6148-8-150
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发表时间：
2012-08-31
影响因子：
2.6
通讯作者：
Arai T
中科院分区：
文献类型：
--
作者：
Takemitsu H;Zhao D;Yamamoto I;Harada Y;Michishita M;Arai T
骨髓源性间充质干细胞（BM-MSC）和脂肪组织源性间充质干细胞（AT-MSC）是治疗性干细胞的潜在细胞来源。MSC是能够分化成许多中胚层谱系的多能细胞群。使用MSC的治疗似乎是再生医学中结构恢复的一种有用方法。这些细胞的正确鉴定是必要的，但在狗的MSC的细胞表面标志物和mRNA表达的资料不足。在这项研究中，我们进行了犬骨髓间充质干细胞和AT-MSCs的分子特征，免疫学和mRNA表达分析。基于其成骨和成脂分化，证实样品是多能的。流式细胞仪检测这些细胞作为干细胞、造血细胞和胚胎干细胞（ESC）标志物。BM-和AT-MSC显示高表达CD 29和CD 44，中等表达CD 90，并且对CD 34、CD 45、SSEA-3、SSEA-4、TRA-1-60和TRA-1-81呈阴性。SSEA-1在AT-MSC中以非常低的水平表达。定量实时PCR（qRT-PCR）显示BM-和AT-MSC中Oct 3/4、Sox 2和Nanog的表达。Oct 3/4和Sox 2在BM-MSCs和AT-MSCs中的表达差异无统计学意义。然而，Nanog在AT-MSCs中的表达是BM-MSCs的2.5倍。通过免疫细胞化学分析，在BM-和AT-MSCs中观察到Oct 3/4和Sox 2蛋白。我们的研究结果提供了基础信息，使更多的可重复性和可靠的质量控制，在犬BM-MSCs和AT-MSCs的蛋白质和mRNA表达分析的鉴定。
Bone marrow-derived mesenchymal stem cells (BM-MSCs) and adipose tissue-derived mesenchymal stem cells (AT-MSCs) are potential cellular sources of therapeutic stem cells. MSCs are a multipotent population of cells capable of differentiating into a number of mesodermal lineages. Treatment using MSCs appears to be a helpful approach for structural restoration in regenerative medicine. Correct identification of these cells is necessary, but there is inadequate information on the MSC profile of cell surface markers and mRNA expression in dogs. In this study, we performed molecular characterization of canine BM-MSCs and AT-MSCs using immunological and mRNA expression analysis. Samples were confirmed to be multipotent based on their osteogenic and adipogenic differentiation. And these cells were checked as stem cell, hematopoietic and embryonic stem cell (ESC) markers by flow cytometry. BM- and AT-MSCs showed high expression of CD29 and CD44, moderate expression of CD90, and were negative for CD34, CD45, SSEA-3, SSEA-4, TRA-1-60, and TRA-1-81. SSEA-1 was expressed at very low levels in AT-MSCs. Quantitative real-time PCR (qRT-PCR) revealed expression of Oct3/4, Sox2, and Nanog in BM- and AT-MSCs. There was no significant difference in expression of Oct3/4 and Sox2 between BM-MSCs and AT-MSCs. However, Nanog expression was 2.5-fold higher in AT-MSCs than in BM-MSCs. Using immunocytochemical analysis, Oct3/4 and Sox2 proteins were observed in BM- and AT-MSCs. Our results provide fundamental information to enable for more reproducible and reliable quality control in the identification of canine BM-MSCs and AT-MSCs by protein and mRNA expression analysis.
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影响因子：
64.5
作者：
Mitsui, K;Tokuzawa, Y;Yamanaka, S
通讯作者：
Yamanaka, S
影响因子：
5.2
作者：
Mitchell, James B.;McIntosh, Kevin;Gimble, Jeffrey M.
通讯作者：
Gimble, Jeffrey M.
影响因子：
5.2
作者：
Katz, AJ;Tholpady, A;Ogle, RC
通讯作者：
Ogle, RC
影响因子：
2.8
作者：
CAPLAN, AI
通讯作者：
CAPLAN, AI
影响因子：
3.6
作者：
Huang, JI;Beanes, SR;Benhaim, P
通讯作者：
Benhaim, P