Diurnal Fluctuations in Plasma Hydrogen Sulfide of the Mice.
Diurnal Fluctuations in Plasma Hydrogen Sulfide of the Mice.
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小鼠血浆硫化氢的昼夜波动
DOI：
10.3389/fphar.2017.00682
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发表时间：
2017
影响因子：
5.6
通讯作者：
Wu Y
中科院分区：
文献类型：
--
作者：
Jin S;Tan B;Teng X;Meng R;Jiao X;Tian D;Xiao L;Xue H;Guo Q;Duan X;Wu Y
昼夜节律在维持体内平衡，特别是氧化还原平衡的无数生理过程中是必不可少的。然而，很少有人知道血浆H2S是否表现出生理昼夜变化。本研究旨在研究血浆H2S浓度的昼夜变化规律并探讨其可能的机制。结果表明，C57 BL/6 J小鼠19时血浆H_2S浓度显著高于7时，且不受采血顺序和血浆半胱氨酸（H_2S前体）浓度的影响。在7：00和19：00之间未观察到CSE、CBS或MPST的mRNA或蛋白表达的显著差异。CSE和CBS活性在19点时差异不显著，而MPST活性在19点时显著高于对照组。AOAA（CBS抑制剂）或PPG（CSE抑制剂）治疗14天后，19点的血浆H2S浓度仍显著高于7点的血浆H2S浓度，但两者均显著低于对照组。在CSE KO小鼠中也观察到相同的发现。19时血浆H_2O_2浓度显著高于7时。而连续光照24 h后，19时H2 O2浓度较7时显著降低。同时，血浆H2S水平和组织MPST活性的昼夜波动消失。DTT治疗14天后，7点和19点的血浆H2 O2浓度无显著差异。同时，血浆H2S水平和组织MPST活性的昼夜波动消失。在SOD 2 +/-小鼠中也观察到相同的结果。体外培养心脏组织，当H_2O_2浓度增加时，在一定浓度范围内，H_2O_2可剂量依赖性地增加MPST的活性。总之，我们的研究表明，血浆H2S浓度和组织MPST活性表现出昼夜波动。MPST活性的变化受血浆H_2O_2浓度的调节，可能导致血浆H_2S的日变化。
Circadian rhythms are essential in a myriad of physiological processes to maintain homeostasis, especially the redox homeostasis. However, little is known about whether plasma H2S exhibits the physiological diurnal variation. The present study was performed to investigate the diurnal fluctuations of plasma H2S and explore the potential mechanisms. We found that the plasma H2S of the C57BL/6J mice was significantly higher at 19 o’clock than those at 7 o’clock which was not affected by the blood-collecting sequence and the concentrations of plasma cysteine (a precursor of H2S). No significant differences in mRNA or protein expression of the CSE, CBS, or MPST were observed between 7: 00 and 19: 00. There were also no significant differences in the CSE and CBS activities, while the activities of MPST in tissues were significantly higher at 19 o’clock. After treatment with AOAA (a CBS inhibitor) or PPG (a CSE inhibitor) for 14 days, plasma H2S concentrations at 19 o’clock were still significantly higher than those at 7 o’clock, although they were both significantly decreased as compared with controls. Identical findings were also observed in CSE KO mice. We also found the plasma H2O2 concentrations were significantly higher at 19 o’clock than those at 7 o’clock. However, H2O2 concentrations were significantly decreased at 19 o’clock than those at 7 o’clock when mice were exposed to continuous light for 24 h. Meanwhile, the diurnal fluctuations of plasma H2S levels and MPST activities in tissues were disappeared. After treatment with DTT for 14 days, there was no significant difference in plasma H2O2 concentrations between 7 o’clock and 19 o’clock. Meanwhile, the diurnal fluctuations of plasma H2S levels and MPST activities in tissues were disappeared. Identical findings were also observed in SOD2+/- mice. When heart tissues were incubated with increasing concentrations of H2O2 in vitro, H2O2 could dose-dependently increase the activity of MPST within a certain concentration range. In conclusion, our studies revealed that plasma H2S concentration and tissue MPST activity exhibited diurnal fluctuations. Modulated by plasma H2O2 concentration, changes of MPST activity probably led to the diurnal fluctuations of plasma H2S.
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DOI：
10.1126/science.aah4967
发表时间：
2016-11-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Panda S
通讯作者：
Panda S
影响因子：
30.3
作者：
Leone V;Gibbons SM;Martinez K;Hutchison AL;Huang EY;Cham CM;Pierre JF;Heneghan AF;Nadimpalli A;Hubert N;Zale E;Wang Y;Huang Y;Theriault B;Dinner AR;Musch MW;Kudsk KA;Prendergast BJ;Gilbert JA;Chang EB
通讯作者：
Chang EB
影响因子：
4.6
作者：
Kimura Y;Toyofuku Y;Koike S;Shibuya N;Nagahara N;Lefer D;Ogasawara Y;Kimura H
通讯作者：
Kimura H
影响因子：
10.5
作者：
Pekovic-Vaughan V;Gibbs J;Yoshitane H;Yang N;Pathiranage D;Guo B;Sagami A;Taguchi K;Bechtold D;Loudon A;Yamamoto M;Chan J;van der Horst GT;Fukada Y;Meng QJ
通讯作者：
Meng QJ
硫化氢通过恢复内皮祖细胞功能和激活 2 型糖尿病患者的血管生成素-1 来改善伤口愈合
影响因子：
7.7
作者：
Liu F;Chen DD;Sun X;Xie HH;Yuan H;Jia W;Chen AF
通讯作者：
Chen AF