Novel organization of mitochondrial minicircles and guide RNAs in the zoonotic pathogen Trypanosoma lewisi.
Novel organization of mitochondrial minicircles and guide RNAs in the zoonotic pathogen Trypanosoma lewisi.
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人畜共患病病原体路易斯锥虫中线粒体小环和引导RNA的新组织
DOI：
10.1093/nar/gkaa700
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发表时间：
2020-09-25
影响因子：
14.9
通讯作者：
Lun ZR
中科院分区：
文献类型：
--
作者：
Li SJ;Zhang X;Lukeš J;Li BQ;Wang JF;Qu LH;Hide G;Lai DH;Lun ZR
摘要动质体鞭毛虫有几个不同寻常的特征，其中之一是其复杂的线粒体基因组，称为动叶绿体(K)DNA，由相互连接的大环和小环组成。路易氏锥虫是锥虫的一种，根据人类感染和实验数据，该种锥虫现在被认为是一种人畜共患病病原体。通过组装总共58个微环类，这些类分为两个不同的类别，我们描述了T.lewiti中一种新型的kDNA组织。RNA-seq方法使我们能够将尿苷插入和删除编辑事件的细节映射到kDNA转录组上。此外，对小RNA分子的测序能够识别169个独特的GUIDE(G)RNA基因，其中两个组织不同的小环类别都编码必要的gRNA。勒维氏锥虫中前所未有的微环和gRNAs的组织拓宽了我们对这些人和动物病原体线粒体基因组的结构和表达的了解。最后，给出了一个描述微圆演化的场景。
Abstract Kinetoplastid flagellates are known for several unusual features, one of which is their complex mitochondrial genome, known as kinetoplast (k) DNA, composed of mutually catenated maxi- and minicircles. Trypanosoma lewisi is a member of the Stercorarian group of trypanosomes which is, based on human infections and experimental data, now considered a zoonotic pathogen. By assembling a total of 58 minicircle classes, which fall into two distinct categories, we describe a novel type of kDNA organization in T. lewisi. RNA-seq approaches allowed us to map the details of uridine insertion and deletion editing events upon the kDNA transcriptome. Moreover, sequencing of small RNA molecules enabled the identification of 169 unique guide (g) RNA genes, with two differently organized minicircle categories both encoding essential gRNAs. The unprecedented organization of minicircles and gRNAs in T. lewisi broadens our knowledge of the structure and expression of the mitochondrial genomes of these human and animal pathogens. Finally, a scenario describing the evolution of minicircles is presented.
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影响因子：
4.5
作者：
Golden, DE;Hajduk, SL
通讯作者：
Hajduk, SL
DOI：
10.1016/0309-1651(88)90050-1
发表时间：
1988-10-01
期刊：
CELL BIOLOGY INTERNATIONAL REPORTS
影响因子：
--
作者：
COSTANZO, G;BIRAGO, C;BATTAGLIA, PA
通讯作者：
BATTAGLIA, PA
影响因子：
1.5
作者：
Briones, MRS;Souto, RP;Zingales, B
通讯作者：
Zingales, B
影响因子：
16
作者：
Aphasizheva I;Maslov D;Wang X;Huang L;Aphasizhev R
通讯作者：
Aphasizhev R
影响因子：
1.5
作者：
BORST, P;VANDERPLOEG, M;JAMES, J
通讯作者：
JAMES, J