The characterisation of pluripotent and multipotent stem cells using Fourier transform infrared microspectroscopy.

The characterisation of pluripotent and multipotent stem cells using Fourier transform infrared microspectroscopy.
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DOI:
10.3390/ijms140917453
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发表时间:
2013-08-26
影响因子:
5.6
通讯作者:
Heraud P
Heraud P
中科院分区:
生物学2区
文献类型:
--
作者:
Cao J;Ng ES;McNaughton D;Stanley EG;Elefanty AG;Tobin MJ;Heraud P

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傅里叶变换红外 (FTIR) 显微光谱显示出作为良性、客观和快速工具筛选临床用途的多能干细胞的潜力。它提供了一种新的实验方法,可以对大分子组成进行整体测量,从而获得代表内部细胞表型的特征。因此,该技术的使用提供了与传统遗传和免疫组织化学方法获得的信息互补的信息。
Fourier transform infrared (FTIR) microspectroscopy shows potential as a benign, objective and rapid tool to screen pluripotent and multipotent stem cells for clinical use. It offers a new experimental approach that provides a holistic measurement of macromolecular composition such that a signature representing the internal cellular phenotype is obtained. The use of this technique therefore contributes information that is complementary to that acquired by conventional genetic and immunohistochemical methods.
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