Optical spectroscopy for noninvasive monitoring of stem cell differentiation.
Optical spectroscopy for noninvasive monitoring of stem cell differentiation.
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DOI：
10.1155/2010/101864
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发表时间：
2010
影响因子：
--
通讯作者：
Elfick A
中科院分区：
文献类型：
--
作者：
Downes A;Mouras R;Elfick A
需要一种非侵入性技术来监测干细胞分化。在这篇综述中讨论了几种基于光学光谱的候选方法：傅里叶变换红外（FTIR）光谱，拉曼光谱和相干反斯托克斯拉曼散射（汽车）显微镜。这些技术进行了简要描述，并讨论了每一个区分未分化的分化细胞的能力。傅立叶变换红外光谱已经证明了其区分干细胞及其衍生物的能力。拉曼光谱显示在胚胎干细胞分化过程中DNA和RNA浓度明显降低（与众所周知的细胞核与细胞质比率降低一致），并且还显示在间充质干细胞分化过程中矿物质含量明显增加。汽车显微镜可以高速绘制这些DNA、RNA和矿物质浓度，而Mulliplex汽车光谱/显微镜被认为是最有前途的未来应用技术。
There is a requirement for a noninvasive technique to monitor stem cell differentiation. Several candidates based on optical spectroscopy are discussed in this review: Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy, and coherent anti-Stokes Raman scattering (CARS) microscopy. These techniques are briefly described, and the ability of each to distinguish undifferentiated from differentiated cells is discussed. FTIR spectroscopy has demonstrated its ability to distinguish between stem cells and their derivatives. Raman spectroscopy shows a clear reduction in DNA and RNA concentrations during embryonic stem cell differentiation (agreeing with the well-known reduction in the nucleus to cytoplasm ratio) and also shows clear increases in mineral content during differentiation of mesenchymal stem cells. CARS microscopy can map these DNA, RNA, and mineral concentrations at high speed, and Mutliplex CARS spectroscopy/microscopy is highlighted as the technique with most promise for future applications.
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