Complete sequencing of the bla(NDM-1)-positive IncA/C plasmid from Escherichia coli ST38 isolate suggests a possible origin from plant pathogens.
Complete sequencing of the bla(NDM-1)-positive IncA/C plasmid from Escherichia coli ST38 isolate suggests a possible origin from plant pathogens.
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DOI：
10.1371/journal.pone.0025334
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Kuroda M
中科院分区：
文献类型：
--
作者：
Sekizuka T;Matsui M;Yamane K;Takeuchi F;Ohnishi M;Hishinuma A;Arakawa Y;Kuroda M
采用大肠埃希菌NDM-1_Dok01（多位点序列分型：ST38）和转偶联大肠埃希菌DH10B对携带新德里金属β-内酰胺酶（NDM-1）的质粒pNDM-1_Dok01进行全基因组鸟枪测序，确定了NDM-1的完整序列。该质粒为IncA/C不相容型，由225个195.5 kb的预测编码序列组成，与大肠杆菌AR060302 pAR060302 （166.5 kb）和沙门氏菌Newport pSN254 （176.4 kb）等bla cmy -2阳性IncA/C质粒部分共享序列。pNDM-1_Dok01中的bla NDM-1基因末端两侧有两个不同于其他NDM-1质粒的IS903元件，这表明bla NDM-1基因与各种可移动元件一起广泛转置，作为盒式基因。在bla NDM-1相关复合转座子中鉴定出了伴蛋白groES和groEL基因，系统发育分析和鸟嘌呤-胞嘧啶含量（GC）百分比与植物病原菌伪黄单胞菌和黄单胞菌等同源物相似，提示植物病原菌可能是bla NDM-1基因的潜在来源。pNDM-1_Dok01的完整序列表明，bla NDM-1基因是通过广泛分布的IncA/C质粒上的新型复合转座子获得的，并转移到大肠杆菌ST38分离物中。
The complete sequence of the plasmid pNDM-1_Dok01 carrying New Delhi metallo-β-lactamase (NDM-1) was determined by whole genome shotgun sequencing using Escherichia coli strain NDM-1_Dok01 (multilocus sequence typing type: ST38) and the transconjugant E. coli DH10B. The plasmid is an IncA/C incompatibility type composed of 225 predicted coding sequences in 195.5 kb and partially shares a sequence with bla CMY-2-positive IncA/C plasmids such as E. coli AR060302 pAR060302 (166.5 kb) and Salmonella enterica serovar Newport pSN254 (176.4 kb). The bla NDM-1 gene in pNDM-1_Dok01 is terminally flanked by two IS903 elements that are distinct from those of the other characterized NDM-1 plasmids, suggesting that the bla NDM-1 gene has been broadly transposed, together with various mobile elements, as a cassette gene. The chaperonin groES and groEL genes were identified in the bla NDM-1-related composite transposon, and phylogenetic analysis and guanine-cytosine content (GC) percentage showed similarities to the homologs of plant pathogens such as Pseudoxanthomonas and Xanthomonas spp., implying that plant pathogens are the potential source of the bla NDM-1 gene. The complete sequence of pNDM-1_Dok01 suggests that the bla NDM-1 gene was acquired by a novel composite transposon on an extensively disseminated IncA/C plasmid and transferred to the E. coli ST38 isolate.
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