Uranium speciation in biofilms studied by laser fluorescence techniques

Uranium speciation in biofilms studied by laser fluorescence techniques
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通过激光荧光技术研究生物膜中的铀形态

DOI:
10.1007/s00216-009-3296-5
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发表时间:
2010
影响因子:
4.3
通讯作者:
Baumann N.
Baumann N.
中科院分区:
化学2区
文献类型:
--
作者:
Arnold T;Großmann K;Baumann N.

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生物膜可以吸附环境中的有毒重金属,从而影响其迁移行为。这些过程的机制目前尚不清楚,因为这种生物膜的复杂性产生了许多离散的地球化学微环境,这些微环境可能与周围的本体溶液在细菌多样性、其普遍的地球化学性质(例如pH和溶解氧浓度)、有机分子(例如代谢物)的存在等方面不同,所有这些都可能影响金属形态。为了获得这样的信息,这是必要的性能评估研究或新的成本效益的战略,清洁废水沃茨的发展,这是非常重要的是开发新的非侵入性的方法,适用于研究生物膜系统内的金属的相互作用。激光荧光技术具有一些上级特点,尤其是对荧光重金属的灵敏度非常高。提出了一种结合激光共聚焦扫描显微镜和激光诱导荧光光谱研究铀与生物膜相互作用的方法。结果发现,耦合这些技术的生物膜系统内的荧光重金属的原位非侵入性研究是一个很有前途的工具。图通过共聚焦激光扫描显微镜(CLSM)和激光诱导荧光光谱(LIFS)的组合,可以在共聚焦体积内可视化和光谱识别光谱信息,例如不同的氧化态。
Biofilms may immobilize toxic heavy metals in the environment and thereby influence their migration behaviour. The mechanisms of these processes are currently not understood, because the complexity of such biofilms creates many discrete geochemical microenvironments which may differ from the surrounding bulk solution in their bacterial diversity, their prevailing geochemical properties, e.g. pH and dissolved oxygen concentration, the presence of organic molecules, e.g. metabolites, and many more, all of which may affect metal speciation. To obtain such information, which is necessary for performance assessment studies or the development of new cost-effective strategies for cleaning waste waters, it is very important to develop new non-invasive methods applicable to study the interactions of metals within biofilm systems. Laser fluorescence techniques have some superior features, above all very high sensitivity for fluorescent heavy metals. An approach combining confocal laser scanning microscopy and laser-induced fluorescence spectroscopy for study of the interactions of biofilms with uranium is presented. It was found that coupling these techniques furnishes a promising tool for in-situ non-invasive study of fluorescent heavy metals within biofilm systems. Information on uranium speciation and uranium redox states can be obtained.FigureSpectroscopic information, e.g. different oxidation states, can be visualized and spectroscopically identified within a confocal volume by a combination of confocal laser scanning microscopy (CLSM) and laser-induced fluorescence spectroscopy (LIFS)
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