Uranium speciation in biofilms studied by laser fluorescence techniques
Uranium speciation in biofilms studied by laser fluorescence techniques
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通过激光荧光技术研究生物膜中的铀形态
DOI:
10.1007/s00216-009-3296-5
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发表时间:
2010
影响因子:
4.3
通讯作者:
Baumann N.
中科院分区:
文献类型:
--
作者:
Arnold T;Großmann K;Baumann N.
Biofilms may immobilize toxic heavy metals in the environment and thereby influence their migration behaviour. The mechanisms of these processes are currently not understood, because the complexity of such biofilms creates many discrete geochemical microenvironments which may differ from the surrounding bulk solution in their bacterial diversity, their prevailing geochemical properties, e.g. pH and dissolved oxygen concentration, the presence of organic molecules, e.g. metabolites, and many more, all of which may affect metal speciation. To obtain such information, which is necessary for performance assessment studies or the development of new cost-effective strategies for cleaning waste waters, it is very important to develop new non-invasive methods applicable to study the interactions of metals within biofilm systems. Laser fluorescence techniques have some superior features, above all very high sensitivity for fluorescent heavy metals. An approach combining confocal laser scanning microscopy and laser-induced fluorescence spectroscopy for study of the interactions of biofilms with uranium is presented. It was found that coupling these techniques furnishes a promising tool for in-situ non-invasive study of fluorescent heavy metals within biofilm systems. Information on uranium speciation and uranium redox states can be obtained.FigureSpectroscopic information, e.g. different oxidation states, can be visualized and spectroscopically identified within a confocal volume by a combination of confocal laser scanning microscopy (CLSM) and laser-induced fluorescence spectroscopy (LIFS)
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DOI:
--
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1991
期刊:
影响因子:
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作者:
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通讯作者:
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DOI:
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通讯作者:
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影响因子:
56.9
作者:
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通讯作者:
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DOI:
10.1016/j.nimb.2005.01.069
发表时间:
2005-04-01
影响因子:
1.3
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