Impact of human adipose tissue-derived stem cells on dermatofibrosarcoma protuberans cells in an indirect co-culture: an in vitro study.
Impact of human adipose tissue-derived stem cells on dermatofibrosarcoma protuberans cells in an indirect co-culture: an in vitro study.
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间接共培养中人脂肪组织干细胞对隆突性皮肤纤维肉瘤细胞的影响：一项体外研究
DOI：
10.1186/s13287-021-02512-5
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发表时间：
2021-08-06
影响因子：
7.5
通讯作者：
Sun D
中科院分区：
文献类型：
--
作者：
Yuan Z;Zhu Z;Zhu F;Ding F;Wang Y;Wang X;Luo X;Yang J;Liu F;Sun D
隆突性皮肤纤维肉瘤（DFSP）是除卡波西肉瘤外最常见的皮肤软组织肉瘤，切除后可进行自体脂肪组织移植以满足美容需要。细胞辅助脂肪移植的再生效果取决于脂肪组织来源的干细胞（ADSC）的存在。这是第一个研究，以评估潜在的肿瘤风险，脂肪干细胞可能无意中被定位在附近的肿瘤微环境的DFSP cells.MethodsPrimary DFSP细胞间接共培养与脂肪干细胞在条件培养基或在Transwell系统。通过评估增殖、迁移、侵袭、血管生成和肿瘤相关基因和蛋白来分析影响。这些试验的结果进行了比较之间的共培养和单一culture conditions.ResultsOur实验结果表明，ADSCs能够促进DFSP细胞的增殖，迁移，侵袭，和血管生成;这伴随着β型血小板衍生生长因子受体、I型胶原α 1链、血管内皮生长因子、肝细胞生长因子和碱性成纤维细胞生长因子。ConclusionsThe目前的报告清楚地表明，脂肪干细胞可以增强不同的恶性性质的DFSP细胞在体外，这不应该被忽视时，考虑临床使用的人脂肪干细胞及其相关衍生物在皮肤再生疗法。
BackgroundAutologous adipose tissue transfer may be performed for aesthetic needs following the resection of dermatofibrosarcoma protuberans (DFSP), the most common cutaneous soft tissue sarcoma, excluding Kaposi sarcoma. The regenerative effectiveness of cell-assisted lipotransfer is dependent on the presence of adipose tissue-derived stem cells (ADSCs). This is the first study to evaluate the potential oncological risks as ADSCs could unintentionally be sited within the proximity of the tumor microenvironment of DFSP cells.MethodsPrimary DFSP cells were indirectly co-cultured with ADSCs in a conditioned medium or in a Transwell system. The impact was analyzed by assessing proliferation, migration, invasion, angiogenesis, and tumor-associated genes and proteins. Results of these assays were compared between co-culture and mono-culture conditions.ResultsOur experimental results showed that ADSCs were able to promote proliferation, migration, invasion, and angiogenesis of DFSP cells; this was accompanied by a significant increase in the expression levels of beta-type platelet-derived growth factor receptor, collagen type I alpha 1 chain, vascular endothelial growth factor, hepatocyte growth factor, and basic fibroblast growth factor.ConclusionsThe current report clearly demonstrates that ADSCs can enhance different malignant properties of DFSP cells in vitro, which should not be neglected when considering the clinical use of human ADSCs and its related derivatives in skin regenerative therapies.
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影响因子：
5.1
作者：
Iser, Isabele C.;Ceschini, Stefanie M.;Wink, Marcia R.
通讯作者：
Wink, Marcia R.
影响因子：
13.8
作者：
Criscione, Vincent D.;Weinstock, Martin A.
通讯作者：
Weinstock, Martin A.
影响因子：
15.9
作者：
Abramsson, A;Lindblom, P;Betsholtz, C
通讯作者：
Betsholtz, C
DOI：
10.1016/j.ijbiomac.2021.02.131
发表时间：
2021-02-24
影响因子：
8.2
作者：
Fathi, Ezzatollah;Farahzadi, Raheleh;Valipour, Behnaz
通讯作者：
Valipour, Behnaz
影响因子：
2.6
作者：
Fathi, Ezzatollah;Farahzadi, Raheleh;Vietor, Ilja
通讯作者：
Vietor, Ilja