Macroporous nanowire nanoelectronic scaffolds for synthetic tissues.
Macroporous nanowire nanoelectronic scaffolds for synthetic tissues.
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作者：
三维（3D）合成生物材料作为结构和生物活性支架的发展是从细胞生物物理学到再生医学等领域的核心。到目前为止，这些支架还不能在其3D和大孔内部电探测物理化学和生物微环境，尽管这种能力可能对电子和生物材料产生显着影响。在这里，我们使用大孔，灵活和独立的纳米线纳米电子支架（nanoES），以及它们与合成或天然生物材料的混合物来解决这一挑战。三维大孔纳米ES模拟天然组织支架的结构，它们是通过具有内置应变的共面网状网络的自组织和通过操纵二维网格矩阵形成的。NanoES具有强大的电子特性，并已单独使用或与其他生物材料组合作为生物相容性细胞外支架，用于神经元，心肌细胞和平滑肌细胞的3D培养。此外，我们通过实时监测（i）3D nanoES/心肌细胞结构内的局部电活动，（ii）基于3D nanoES的神经和心脏组织模型对药物的响应，以及（iii）管状血管平滑肌结构内外不同的pH值变化，显示了nanoES的综合感觉能力。
The development of three-dimensional (3D) synthetic biomaterials as structural and bioactive scaffolds is central to fields ranging from cellular biophysics to regenerative medicine. As of yet, these scaffolds cannot electrically probe the physicochemical and biological micro-environments throughout their 3D and macroporous interior, although this capability could have a marked impact in both electronics and biomaterials. Here, we address this challenge using macroporous, flexible and free-standing nanowire nanoelectronic scaffolds (nanoES), and their hybrids with synthetic or natural biomaterials. 3D macroporous nanoES mimic the structure of natural tissue scaffolds, and they were formed by self-organization of coplanar reticular networks with built-in strain and by manipulation of 2D mesh matrices. NanoES exhibited robust electronic properties and have been used alone or combined with other biomaterials as biocompatible extracellular scaffolds for 3D culture of neurons, cardiomyocytes and smooth muscle cells. Additionally, we show the integrated sensory capability of the nanoES by real-time monitoring of (i) the local electrical activity within 3D nanoES/cardiomyocyte constructs, (ii) the response of 3D nanoES based neural and cardiac tissue models to drugs, and (iii) distinct pH changes inside and outside tubular vascular smooth muscle constructs.
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影响因子：
4.8
作者：
L'Heureux, N;Pâquet, S;Auger, FA
通讯作者：
Auger, FA
影响因子：
48
作者：
Kraehenbuehl, Thomas P.;Langer, Robert;Ferreira, Lino S.
通讯作者：
Ferreira, Lino S.
影响因子：
56.9
作者：
Kim, Dae-Hyeong;Lu, Nanshu;Rogers, John A.
通讯作者：
Rogers, John A.
影响因子：
38.3
作者：
通讯作者：
--
DOI：
10.1126/science.1188302
发表时间：
2010-06-25
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Huh D;Matthews BD;Mammoto A;Montoya-Zavala M;Hsin HY;Ingber DE
通讯作者：
Ingber DE