The effect of gut microbiota elimination in Drosophila melanogaster: A how-to guide for host-microbiota studies.
The effect of gut microbiota elimination in Drosophila melanogaster: A how-to guide for host-microbiota studies.
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DOI：
10.1002/ece3.3991
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发表时间：
2018-04
影响因子：
2.6
通讯作者：
Lewis ZJ
中科院分区：
文献类型：
--
作者：
Heys C;Lizé A;Blow F;White L;Darby A;Lewis ZJ
近年来，人们对微生物群对宿主的影响的兴趣激增。我们越来越了解肠道微生物群在调节宿主生理、生态、行为和进化方面的重要性。用于评估微生物群的影响的一种方法是抑制或消除它，并将对宿主的影响与未治疗个体的影响进行比较。在这项研究中，我们评估了一些这些常用的方法在模式生物，果蝇。我们在完全析因设计中测试了低剂量链霉素饮食、鸡蛋脱氯和无菌或无菌饮食在去除该物种肠道细菌方面的功效。我们进一步确定潜在的副作用，这些方法对宿主生理学进行了一系列标准的生理测定。我们的研究结果表明，与正常苍蝇相比，所有处理的个体需要更长的时间才能发育，并且体重更轻。雄性和雌性经历了鸡蛋脱绒的体重显着低于链霉素饲养的个人。同样，纯雌性果蝇，而不是男性，在运动测定分析时，活动少得多。所有方法都降低了卵至成虫的存活率，其中卵脱绒诱导显著更高的死亡率。我们得出结论，在饮食介质中添加低剂量链霉素比鸡蛋脱氯更有效地去除肠道细菌，对宿主生理学的不利影响也较小。更重要的是，这种方法在行为研究中是最实用和可靠的。我们的研究提出了一个重要的问题，即这些方法的有效性和对宿主的影响需要以个案的方式进行调查，而不是假设物种和实验室之间的同质性。
In recent years, there has been a surge in interest in the effects of the microbiota on the host. Increasingly, we are coming to understand the importance of the gut microbiota in modulating host physiology, ecology, behavior, and evolution. One method utilized to evaluate the effect of the microbiota is to suppress or eliminate it, and compare the effect on the host with that of untreated individuals. In this study, we evaluate some of these commonly used methods in the model organism, Drosophila melanogaster. We test the efficacy of a low‐dose streptomycin diet, egg dechorionation, and an axenic or sterile diet, in the removal of gut bacteria within this species in a fully factorial design. We further determine potential side effects of these methods on host physiology by performing a series of standard physiological assays. Our results showed that individuals from all treatments took significantly longer to develop, and weighed less, compared to normal flies. Males and females that had undergone egg dechorionation weighed significantly less than streptomycin reared individuals. Similarly, axenic female flies, but not males, were much less active when analyzed in a locomotion assay. All methods decreased the egg to adult survival, with egg dechorionation inducing significantly higher mortality. We conclude that low‐dose streptomycin added to the dietary media is more effective at removing the gut bacteria than egg dechorionation and has somewhat less detrimental effects to host physiology. More importantly, this method is the most practical and reliable for use in behavioral research. Our study raises the important issue that the efficacy of and impacts on the host of these methods require investigation in a case‐by‐case manner, rather than assuming homogeneity across species and laboratories.
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影响因子：
3.7
作者：
Ridley EV;Wong AC;Westmiller S;Douglas AE
通讯作者：
Douglas AE
影响因子：
3.3
作者：
Kristensen, Torsten N.;Overgaard, Johannes;Sgro, Carla
通讯作者：
Sgro, Carla
DOI：
10.1073/pnas.1009906107
发表时间：
2010-11-16
影响因子：
11.1
作者：
Sharon, Gil;Segal, Daniel;Rosenberg, Eugene
通讯作者：
Rosenberg, Eugene
影响因子：
2.2
作者：
BIEMONT, JC;CHAUVIN, G;HAMON, C
通讯作者：
HAMON, C
影响因子：
6.4
作者：
Broderick NA;Buchon N;Lemaitre B
通讯作者：
Lemaitre B