Impact of the resident microbiota on the nutritional phenotype of Drosophila melanogaster.
Impact of the resident microbiota on the nutritional phenotype of Drosophila melanogaster.
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DOI：
10.1371/journal.pone.0036765
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Douglas AE
中科院分区：
文献类型：
--
作者：
Ridley EV;Wong AC;Westmiller S;Douglas AE
动物长期受到良性有益微生物的感染，这些微生物通常通过对宿主的营养、免疫功能和其他生理系统的影响来促进动物健康。通过比较实验中失去微生物区系的动物和未经处理的动物的特征，可以深入了解宿主与微生物的相互作用。黑腹果蝇是研究宿主-微生物相互作用的一个实验上容易处理的系统。对含有未操纵微生物区系的黑腹果蝇的微生物区系的营养意义进行了研究，通过对16SRRNA扩增片段的454测序证实了微生物区系由α蛋白细菌所控制，并通过鸡蛋脱色实验去除了微生物区系(分别为常规果蝇和无菌果蝇)。在无菌果蝇中，与常规果蝇相比，幼虫发育速度受到抑制，对成虫大小没有影响，表明微生物区系促进了幼虫的生长速度。雌性果蝇的繁殖力在常规果蝇和无菌果蝇之间没有显著差异，但无菌果蝇的代谢率显著降低，碳水化合物分配发生变化，包括血糖水平升高。我们已经证明，消除寄居的微生物区系延长了幼虫的发育，并扰乱了黑腹潜蝇的能量动态平衡和碳水化合物分配模式。我们的结果表明，寄生的微生物区系促进了寄主的营养，并与寄主代谢的调节相互作用。
Animals are chronically infected by benign and beneficial microorganisms that generally promote animal health through their effects on the nutrition, immune function and other physiological systems of the host. Insight into the host-microbial interactions can be obtained by comparing the traits of animals experimentally deprived of their microbiota and untreated animals. Drosophila melanogaster is an experimentally tractable system to study host-microbial interactions. The nutritional significance of the microbiota was investigated in D. melanogaster bearing unmanipulated microbiota, demonstrated by 454 sequencing of 16S rRNA amplicons to be dominated by the α-proteobacterium Acetobacter, and experimentally deprived of the microbiota by egg dechorionation (conventional and axenic flies, respectively). In axenic flies, larval development rate was depressed with no effect on adult size relative to conventional flies, indicating that the microbiota promotes larval growth rates. Female fecundity did not differ significantly between conventional and axenic flies, but axenic flies had significantly reduced metabolic rate and altered carbohydrate allocation, including elevated glucose levels. We have shown that elimination of the resident microbiota extends larval development and perturbs energy homeostasis and carbohydrate allocation patterns of of D. melanogaster. Our results indicate that the resident microbiota promotes host nutrition and interacts with the regulation of host metabolism.
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影响因子：
3.4
作者：
BAKULA, M
通讯作者：
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影响因子：
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作者：
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