Eleven novel microsatellite markers for the Chinese alligator (Alligator sinensis)

Eleven novel microsatellite markers for the Chinese alligator (Alligator sinensis)
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扬子鳄 (Alligator sinensis) 的 11 个新型微卫星标记

DOI:
10.1007/s10592-008-9561-x
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发表时间:
2009-06
影响因子:
2.2
通讯作者:
Lan, Hong
Lan, Hong
中科院分区:
环境科学与生态学3区
文献类型:
--
作者:
Jing, Wei;Fang, Sheng-Guo;Wang, Xiao-Liang;Lan, Hong

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扬子鳄(Alligator sinensis)是我国特有的极危物种。本研究针对这一珍稀物种开发了11个新的微卫星位点,并利用这些微卫星位点分析了长兴自然保护区土著扬子鳄和美洲扬子鳄的遗传变异。11个多态性微卫星在57个个体中共检测到31个等位基因,平均每个位点产生2.82个等位基因。一个等位基因是美国人特有的,但在长兴人中检测到四个私人等位基因。长兴扬子鳄的平均期望杂合度和观察杂合度分别为0.400和0.482,美国出生个体的平均期望杂合度和观察杂合度分别为0.520和0.621。这些微卫星标记将为该濒危物种的遗传学研究提供有用的工具。
Chinese alligator (Alligator sinensis) is a critically endangered species endemic to China. In this study, we developed 11 novel microsatellite loci for this rare species and applied them to examine genetic variation of indigenous alligators from Changxing Nature Reserve and America-born Chinese alligators. The 11 polymorphic microsatellites presented a total of 31 alleles among 57 individuals scored, yielding an average of 2.82 alleles per locus. One allele was unique to the American population but four private alleles were detected in the Changxing population. The average expected and observed heterozygosities were 0.400 and 0.482 for the Changxing alligators and 0.520 and 0.621 for the America-born individuals, respectively. These microsatellite markers would be useful tools in the genetic examination of this endangered species.
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发表时间: 2002
影响因子: 5.9
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