Characterization of germ cell differentiation in the male mouse through single-cell RNA sequencing.
Characterization of germ cell differentiation in the male mouse through single-cell RNA sequencing.
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DOI：
10.1038/s41598-018-24725-0
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发表时间：
2018-04-25
影响因子：
4.6
通讯作者：
Winterpacht A
中科院分区：
文献类型：
--
作者：
Lukassen S;Bosch E;Ekici AB;Winterpacht A
小鼠的精子发生已经被广泛研究了几十年。以前的方法，如组织学染色或批量转录组分析，要么缺乏单细胞水平的分辨率，要么集中在一组非常狭窄的因素。在这里，我们提出了第一个全面的，公正的单细胞转录组学的观点小鼠精子发生。我们对来自小鼠睾丸的2，500多个细胞的单细胞RNA-seq（scRNA-seq）数据改进了阶段标记物检测和验证，捕获了分化的连续性，而不是人为选择的阶段。scRNA-seq还可以分析大量测序数据中隐藏的罕见细胞群体，并揭示了对精子发生过程中性染色体调控的新见解。我们的数据为该领域的进一步研究提供了基础，首次提供了小鼠精子发生过程中转录过程的高分辨率参考。
Spermatogenesis in the mouse has been extensively studied for decades. Previous methods, such as histological staining or bulk transcriptome analysis, either lacked resolution at the single-cell level or were focused on a very narrowly defined set of factors. Here, we present the first comprehensive, unbiased single-cell transcriptomic view of mouse spermatogenesis. Our single-cell RNA-seq (scRNA-seq) data on over 2,500 cells from the mouse testis improves upon stage marker detection and validation, capturing the continuity of differentiation rather than artificially chosen stages. scRNA-seq also enables the analysis of rare cell populations masked in bulk sequencing data and reveals new insights into the regulation of sex chromosomes during spermatogenesis. Our data provide the basis for further studies in the field, for the first time providing a high-resolution reference of transcriptional processes during mouse spermatogenesis.
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