Proteomic Analysis Reveals the Positive Roles of the Plant-Growth-Promoting Rhizobacterium NSY50 in the Response of Cucumber Roots to Fusarium oxysporum f. sp. cucumerinum Inoculation.
Proteomic Analysis Reveals the Positive Roles of the Plant-Growth-Promoting Rhizobacterium NSY50 in the Response of Cucumber Roots to Fusarium oxysporum f. sp. cucumerinum Inoculation.
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蛋白质组学分析揭示了促进植物生长的根际细菌 NSY50 在黄瓜根对尖镰孢 f 反应中的积极作用。
DOI：
10.3389/fpls.2016.01859
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发表时间：
2016
影响因子：
5.6
通讯作者：
Guo S
中科院分区：
文献类型：
--
作者：
Du N;Shi L;Yuan Y;Li B;Shu S;Sun J;Guo S
植物根际促生菌（PGPR）既能促进植物生长，又能增强植物对各种环境胁迫的抵抗力。为探讨PGPR保护植物免受病原菌侵害的机制，采用透射电镜和蛋白质组学方法研究了PGPR新菌株多粘类芽孢杆菌（Paenibacillus polymyxa）NSY 50对黄瓜幼苗接种枯萎病菌后根系的影响。黄瓜黑腐病菌（FOC）。NSY 50可以明显减轻FOC感染引起的损伤，维持细胞结构的稳定。结合MALDI-TOF/TOF分析的双向电泳（2-DE）方法揭示了响应于NSY 50和/或FOC的总共56个差异表达的蛋白。NSY 50的应用上调了大多数已鉴定的参与正常条件下碳水化合物代谢和氨基酸代谢的蛋白质，这意味着能量产生和氨基酸的产生都得到了增强，从而保证了黄瓜幼苗合成新蛋白质所需的充足氨基酸供应，促进了植物生长。接种FOC抑制了大多数与碳水化合物和能量代谢以及蛋白质代谢相关的蛋白质。复合接种（NSY 50 + FOC）积累了丰富的蛋白质，这些蛋白质参与了氧化、解毒和碳水化合物代谢等防御机制，可能在防御病原菌侵袭中发挥重要作用。同时，采用蛋白质印迹法分析烯醇化酶（ENO）和S-腺苷甲硫氨酸合成酶（SAMs）的积累。NSY 50进一步增加FOC胁迫下ENO和SAMs的表达。此外，NSY 50还调节与这些蛋白质相关的基因的转录水平。这些结果表明，多粘原杆菌NSY 50可能通过改善黄瓜根系防御相关蛋白的代谢和活化，促进植物生长，减轻FOC诱导的伤害。
Plant-growth-promoting rhizobacteria (PGPR) can both improve plant growth and enhance plant resistance against a variety of environmental stresses. To investigate the mechanisms that PGPR use to protect plants under pathogenic attack, transmission electron microscopy analysis and a proteomic approach were designed to test the effects of the new potential PGPR strain Paenibacillus polymyxa NSY50 on cucumber seedling roots after they were inoculated with the destructive phytopathogen Fusarium oxysporum f. sp. cucumerinum (FOC). NSY50 could apparently mitigate the injury caused by the FOC infection and maintain the stability of cell structures. The two-dimensional electrophoresis (2-DE) approach in conjunction with MALDI-TOF/TOF analysis revealed a total of 56 proteins that were differentially expressed in response to NSY50 and/or FOC. The application of NSY50 up-regulated most of the identified proteins that were involved in carbohydrate metabolism and amino acid metabolism under normal conditions, which implied that both energy generation and the production of amino acids were enhanced, thereby ensuring an adequate supply of amino acids for the synthesis of new proteins in cucumber seedlings to promote plant growth. Inoculation with FOC inhibited most of the proteins related to carbohydrate and energy metabolism and to protein metabolism. The combined inoculation treatment (NSY50+FOC) accumulated abundant proteins involved in defense mechanisms against oxidation and detoxification as well as carbohydrate metabolism, which might play important roles in preventing pathogens from attacking. Meanwhile, western blotting was used to analyze the accumulation of enolase (ENO) and S-adenosylmethionine synthase (SAMs). NSY50 further increased the expression of ENO and SAMs under FOC stress. In addition, NSY50 adjusted the transcription levels of genes related to those proteins. Taken together, these results suggest that P. polymyxa NSY50 may promote plant growth and alleviate FOC-induced damage by improving the metabolism and activation of defense-related proteins in cucumber roots.
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影响因子：
6.7
作者：
Hong, Chi Eun;Kwon, Suk Yoon;Park, Jeong Mee
通讯作者：
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影响因子：
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作者：
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影响因子：
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影响因子：
4.8
作者：
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影响因子：
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