Tension sensing by Aurora B kinase is independent of survivin-based centromere localization.
Tension sensing by Aurora B kinase is independent of survivin-based centromere localization.
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作者：
复制基因组的精确分离需要染色体在纺锤体上的双向定位。双定向由Aurora B激酶确保，Aurora B激酶是4亚基染色体乘客复合物（CPC）的成员。CPC定位于内部着丝粒是当前模型的核心，该模型用于确定张力如何确保不在张力下的染色体生物取向-动粒-纺锤体附件保持靠近内部着丝粒，并且通过极光B磷酸化而不稳定，而在张力下的动粒被拉离极光B的影响，稳定它们的微管附件。在这里，我们表明，工程截断的INCENP/Sli 15亚基的芽殖酵母CPC，消除与内部着丝粒，但支持适当的染色体分离在有丝分裂和减数分裂。截短的INCENP/Sli 15抑制内着丝粒靶向蛋白Survivin/Bir 1、Borealin/Nbl 1、Bub 1和Sgo 1的缺失表型。与野生型INCENP/Sli 15不同，截短的INCENP/Sli 15定位于后期前纺锤体微管。通过阻止Cdk 1磷酸化将全长INCENP/Sli 15过早靶向微管也抑制了Survivin/Bir 1缺失的不活性。这些结果表明，激活极光B/Ipl 1的染色质或微管上的集群是足够的染色体双取向。
Accurate segregation of the replicated genome requires chromosome biorientation on the spindle. Biorientation is ensured by Aurora B kinase, a member of the 4-subunit chromosomal passenger complex (CPC). Localization of the CPC to the inner centromere is central to the current model for how tension ensures chromosome biorientation—kinetochore-spindle attachments not under tension remain close to the inner centromere and are destabilized by Aurora B phosphorylation, whereas kinetochores under tension are pulled away from the influence of Aurora B, stabilizing their microtubule attachments. Here we show that an engineered truncation of the INCENP/Sli15 subunit of budding yeast CPC that eliminates association with the inner centromere nevertheless supports proper chromosome segregation during both mitosis and meiosis. Truncated INCENP/Sli15 suppresses the deletion phenotypes of the inner centromere-targeting proteins Survivin/Bir1, Borealin/Nbl1, Bub1 and Sgo1. Unlike wildtype INCENP/Sli15, truncated INCENP/Sli15 localizes to pre-anaphase spindle microtubules. Premature targeting of full-length INCENP/Sli15 to microtubules by preventing Cdk1 phosphorylation also suppresses inviability of Survivin/Bir1 deletion. These results suggest that activation of Aurora B/Ipl1 by clustering either on chromatin or on microtubules is sufficient for chromosome biorientation.
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影响因子：
16.8
作者：
Cho, Uhn-Soo;Harrison, Stephen C.
通讯作者：
Harrison, Stephen C.
影响因子：
3.3
作者：
Shimogawa, Michelle M.;Widlund, Per O.;Davis, Trisha N.
通讯作者：
Davis, Trisha N.
影响因子：
11.8
作者：
Tseng, Boo Shan;Tan, Lei;Funabiki, Hironori
通讯作者：
Funabiki, Hironori
影响因子：
64.5
作者：
LI, R;MURRAY, AW
通讯作者：
MURRAY, AW
DOI：
10.1126/science.1189435
发表时间：
2010-10-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Wang F;Dai J;Daum JR;Niedzialkowska E;Banerjee B;Stukenberg PT;Gorbsky GJ;Higgins JM
通讯作者：
Higgins JM