The endophytic bacterium, Sphingomonas SaMR12, improves the potential for zinc phytoremediation by its host, Sedum alfredii.
The endophytic bacterium, Sphingomonas SaMR12, improves the potential for zinc phytoremediation by its host, Sedum alfredii.
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内生细菌鞘氨醇单胞菌 SaMR12 提高了其宿主景天的锌植物修复潜力
DOI：
10.1371/journal.pone.0106826
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Feng Y
中科院分区：
文献类型：
--
作者：
Chen B;Shen J;Zhang X;Pan F;Yang X;Feng Y
从东南景天（Sedum alfredii Hance）中分离的内生细菌鞘氨醇单胞菌（SphingomonasSaMR 12）似乎增加了植物生物量和从污染土壤中提取锌;然而，发生这种情况的机制尚不清楚。在这里，SaMR 12促进锌提取的能力及其对根形态和分泌的影响在水培中进行了研究。锌处理使地上部生物量增加了30%至45%，当与SaMR 12接种结合时进一步增加了10%至19%。锌处理也适度增加了锌的积累，SaMR 12也增强了这一点。生物量和锌水平均以剂量依赖性方式增加，在50 µM锌时观察到显著效应，在500 µM时观察到表观饱和。锌和内生菌也增加了生长素的水平，但不是在50 µM，锌增加了超氧化物和过氧化氢的水平，但主要是在500 µM。至于根形态，SaMR 12增加了根的分枝，根尖的数量和表面积。锌和SaMR 12也增加了草酸的渗出。对于大多数试验，内生菌和锌的作用是相加的，但SaMR 12在500 µM锌时强烈减少活性氧的产生是一个明显的例外。总之，这些结果表明，通过暴露于锌和SaMR 12促进生长和锌吸收独立于活性氧，并且不涉及生长素的增加。
The endophytic bacterium, Sphingomonas SaMR12, isolated from Sedum alfredii Hance, appears to increase plant biomass and zinc-extraction from contaminated soil; however, the mechanism by which this occurs is not clear. Here, the ability of SaMR12 to promote zinc extraction and its effects on root morphology and exudation were examined in hydroponics. Zinc treatment increased shoot biomass by 30 to 45%, and by a further 10 to 19% when combined with SaMR12 inoculation. Zinc treatment also increased zinc accumulation modestly and this too was enhanced with SaMR12. Both biomass and zinc levels increased in a dose-dependent manner with significant effects seen at 50 µM zinc and apparent saturation at 500 µM. Zinc and the endophyte also increased levels of auxin but not at 50 µM and zinc increased levels of superoxide and hydrogen peroxide but mainly at 500 µM. As for root morphology, SaMR12 increased root branching, the number of root tips, and surface area. Zinc and SaMR12 also increased the exudation of oxalic acid. For most assays the effects of the endophyte and zinc were additive, with the notable exception of SaMR12 strongly reducing the production of reactive oxygen species at 500 µM zinc. Taken together, these results suggest that the promotion of growth and zinc uptake by exposure to zinc and to SaMR12 are independent of reactive oxygen and do not involve increases in auxin.
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影响因子：
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