Identification of immediate response genes dominantly expressed in juvenile resistant and susceptible Biomphalaria glabrata snails upon exposure to Schistosoma mansoni.
Identification of immediate response genes dominantly expressed in juvenile resistant and susceptible Biomphalaria glabrata snails upon exposure to Schistosoma mansoni.
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DOI：
10.1016/j.molbiopara.2009.09.009
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发表时间：
2010-01
影响因子：
1.5
通讯作者：
Knight, Matty
中科院分区：
文献类型：
--
作者：
Ittiprasert, Wannaporn;Miller, Andre;Myers, Jocelyn;Nene, Vish;El-Sayed, Najib M.;Knight, Matty
关键词：
蜗牛宿主光滑双脐螺对曼氏血吸虫的抗性或易感性由蜗牛和寄生虫的遗传决定。虽然孟德尔遗传学控制成年人对感染的抵抗力，但幼年人的抵抗力和易感性是复杂的性状。本研究利用抑制性消减杂交技术构建了钉螺抗S-90、LAC 2和NMRI的正向和反向cDNA文库，以鉴定与钉螺早期暴露后的即刻反应相关的基因。mansoni表达序列标签（EST）从富集的转录物库产生。在抗性蜗牛中，几个EST对应于参与免疫调节/防御反应的转录本。虽然未发现防御相关的转录子之间的青少年易感蜗牛EST，我们检测到的生物过程/形态发生/增殖负调控的转录子。采用定量真实的时间RT-PCR比较了蜗牛暴露于正常毛蚴或减毒毛蚴前后的代表性转录本的差异基因表达和时间调控。结果表明，无论毛蚴感染与否，钉螺的纤溶蛋白C末端结构域、胞苷脱氨酶、巨噬细胞表达基因1、蛋白激酶C受体、抗菌肽、theromacin和Fas等转录物均呈上调表达。而与C型凝集素和低密度脂蛋白受体相关的ESTs仅在正常毛蚴中诱导表达.通过比较基因表达的变化之间的抗性和敏感的少年蜗牛响应正常或减毒的寄生虫，我们可以得出结论，与蜗牛宿主的入侵毛蚴的皮内渗透过程相关的基因的转录可能需要考虑评估之间的差异基因表达的抗性和敏感菌株的B。glabrata与S.曼索尼暴露。
Resistance or susceptibility of the snail host Biomphalaria glabrata to Schistosoma mansoni is determined by the genetics of both the snail and parasite. Although Mendelian genetics governs adult resistance to infection, juvenile resistance and susceptibility are complex traits. In this study, suppression subtractive hybridization was used to construct forward and reverse cDNA libraries to identify genes involved in the immediate response of juvenile resistant (BS-90), non-susceptible (LAC2) snails, and susceptible (NMRI) snails after early exposure to S. mansoni. Expressed Sequence Tags (ESTs) were generated from the repertoire of enriched transcripts. In resistant snails, several ESTs corresponded to transcripts involved in immune regulation/defense response. While no defense related transcripts were found among juvenile susceptible snail ESTs, we detected transcripts involved in negative regulation of biological process/morphogenesis /proliferation. Differential gene expression and temporal regulation of representative transcripts were compared among snails pre- and post-exposure to either normal or attenuated miracidia using quantitative real time RT-PCR. Results showed that several transcripts, such as fibrinolytic C terminal domain, cytidine deaminase, macrophage expressed gene 1, protein kinase C receptor, anti- microbial peptide; theromacin and Fas remained up- regulated regardless of whether or not snails were exposed to normal or attenuated miracidia. While ESTs related to C- type lectin and low- density lipoprotein receptor were induced only by exposure to normal miracidia. By comparing changes in gene expression between resistant and susceptible juvenile snails responding either to normal or attenuated parasites, we can conclude that the transcription of genes associated with the intra-dermal penetration process of the snail host by invading miracidia may need to be taken into account when assessing differential gene expression between resistant and susceptible strains of B. glabrata in relation to S. mansoni exposure.
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影响因子：
1.3
作者：
Bender, RC;Broderick, EJ;Bayne, CJ
通讯作者：
Bayne, CJ
影响因子：
2.1
作者：
Lockyer, AE;Noble, LR;Jones, CS
通讯作者：
Jones, CS
DOI：
10.1093/bioinformatics/btn615
发表时间：
2009-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Carbon S;Ireland A;Mungall CJ;Shu S;Marshall B;Lewis S;AmiGO Hub;Web Presence Working Group
通讯作者：
Web Presence Working Group
DOI：
10.1073/pnas.94.16.8691
发表时间：
1997-08-05
影响因子：
11.1
作者：
Adema, CM;Hertel, LA;Loker, ES
通讯作者：
Loker, ES
影响因子：
1.5
作者：
Lockyer, Anne E.;Spinks, Jennifer;Jones, Catherine S.
通讯作者：
Jones, Catherine S.