Unraveling the Genetic Basis for the Rapid Diversification of Male Genitalia between Drosophila Species.
Unraveling the Genetic Basis for the Rapid Diversification of Male Genitalia between Drosophila Species.
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DOI：
10.1093/molbev/msaa232
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发表时间：
2021-01-23
影响因子：
10.7
通讯作者：
McGregor AP
中科院分区：
文献类型：
--
作者：
Hagen JFD;Mendes CC;Booth SR;Figueras Jimenez J;Tanaka KM;Franke FA;Baudouin-Gonzalez L;Ridgway AM;Arif S;Nunes MDS;McGregor AP
在过去的240，000年里，果蝇物种进化枝的雄性在其表皮后叶和抱器（surstyli）的形态上进化出了惊人的差异。这些附属物用于在交配时抓住雌性，因此它们的分歧很可能是由性选择驱动的。作图研究表明，这些形态差异的高度多基因和一般加性遗传基础。然而，我们对控制生殖器结构发育的基因调控网络以及它们如何进化以导致这种快速表型多样化的理解有限。在这里，我们在染色体臂3L上使用新的D. simulans/D. mauritiana渐渗系来生成这些物种之间的后叶和抱茎差异的更高分辨率的图谱。然后，我们对这两个物种发育中的生殖器进行了RNA-seq，以确定表达的基因和两个物种之间差异表达的基因。这使我们能够测试表达的位置候选人在黑腹果蝇生殖器发育过程中的功能。我们确定了几个新的基因参与的发展和可能的进化，这些生殖器结构，包括转录因子毛和垃圾。此外，我们发现，在抱茎发育毛负调控格子呢（trn），一个基因有助于分歧的抱茎形态。总的来说，我们的研究结果为生殖器发育的调节以及物种之间的进化提供了新的见解。
In the last 240,000 years, males of the Drosophila simulans species clade have evolved striking differences in the morphology of their epandrial posterior lobes and claspers (surstyli). These appendages are used for grasping the female during mating and so their divergence is most likely driven by sexual selection. Mapping studies indicate a highly polygenic and generally additive genetic basis for these morphological differences. However, we have limited understanding of the gene regulatory networks that control the development of genital structures and how they evolved to result in this rapid phenotypic diversification. Here, we used new D. simulans/D. mauritiana introgression lines on chromosome arm 3L to generate higher resolution maps of posterior lobe and clasper differences between these species. We then carried out RNA-seq on the developing genitalia of both species to identify the expressed genes and those that are differentially expressed between the two species. This allowed us to test the function of expressed positional candidates during genital development in D. melanogaster. We identified several new genes involved in the development and possibly the evolution of these genital structures, including the transcription factors Hairy and Grunge. Furthermore, we discovered that during clasper development Hairy negatively regulates tartan (trn), a gene known to contribute to divergence in clasper morphology. Taken together, our results provide new insights into the regulation of genital development and how this has evolved between species.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
3.3
作者：
Eberhard, William G.
通讯作者：
Eberhard, William G.
影响因子：
3.7
作者：
House CM;Lewis Z;Hodgson DJ;Wedell N;Sharma MD;Hunt J;Hosken DJ
通讯作者：
Hosken DJ
影响因子：
4.6
作者：
Chatterjee, Sujash S.;Uppendahl, Locke D.;Siegal, Mark L.
通讯作者：
Siegal, Mark L.
影响因子：
1.2
作者：
Chandran, Rachana R.;Iordanou, Ekaterini;Jiang, Lan
通讯作者：
Jiang, Lan