Polarization and interaction of adhesion molecules P-selectin glycoprotein ligand 1 and intercellular adhesion molecule 3 with moesin and ezrin in myeloid cells.
Polarization and interaction of adhesion molecules P-selectin glycoprotein ligand 1 and intercellular adhesion molecule 3 with moesin and ezrin in myeloid cells.
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骨髓细胞中粘附分子 P-选择素糖蛋白配体 1 和细胞间粘附分子 3 的极化和与 moesin 和 ezrin 的相互作用。
DOI：
10.1182/blood.v95.7.2413
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发表时间：
2000
期刊：
影响因子：
20.3
通讯作者：
F. Sánchez‐Madrid
中科院分区：
文献类型：
--
作者：
J. Alonso;J. Serrador;C. Domı́nguez;O. Barreiro;A. Luque;M. A. del Pozo;K. Snapp;G. Kansas;R. Schwartz;H. Furthmayr;F. Lozano;F. Sánchez‐Madrid
黏附分子P-选择素糖蛋白配体1(PSGL-1)、细胞间黏附分子3(ICAM-3)、CD43和CD44在趋化因子白介素8、C5a、N-甲酰甲硫基亮氨酸苯丙氨酸和白介素15的作用下，重新分布到人中性粒细胞新形成的尿荚中。黏附分子PSGL-1和ICAM-3与细胞骨架蛋白moesin在刺激后的中性粒细胞中共定位。在HL-60细胞裂解物中，通过分离PSGL-1胞浆结构域的谷胱甘肽S-转移酶融合的复合体，证实了PSGL-1与Moesin的相互作用。蛋白质印迹分析表明，78-kd和81-kd的条带分别为moesin和ezrin。用抗ICAM-3免疫亲和试验从中性粒细胞裂解液中洗脱出ICAM-3和Moesin。蛋白质结合实验表明，ICAM-3和PSGL-1的胞质结构域与重组Moesin的氨基末端结构域直接相互作用。这些结果表明，PSGL-1的重新分布及其与细胞内分子，包括Ezrin-Radioxin-moesin肌动蛋白结合蛋白的结合，调节了PSGL-1在化学诱导剂刺激的白细胞中介导的功能。
In response to the chemoattractants interleukin 8, C5a, N-formyl-methionyl-leucyl-phenylalanine, and interleukin 15, adhesion molecules P-selectin glycoprotein ligand 1 (PSGL-1), intercellular adhesion molecule 3 (ICAM-3), CD43, and CD44 are redistributed to a newly formed uropod in human neutrophils. The adhesion molecules PSGL-1 and ICAM-3 were found to colocalize with the cytoskeletal protein moesin in the uropod of stimulated neutrophils. Interaction of PSGL-1 with moesin was shown in HL-60 cell lysates by isolating a complex with glutathione S-transferase fusions of the cytoplasmic domain of PSGL-1. Bands of 78- and 81-kd were identified as moesin and ezrin by Western blot analysis. ICAM-3 and moesin also coeluted from neutrophil lysates with an anti-ICAM-3 immunoaffinity assay. Direct interaction of the cytoplasmic domains of ICAM-3 and PSGL-1 with the amino-terminal domain of recombinant moesin was demonstrated by protein-protein binding assays. These results suggest that the redistribution of PSGL-1 and its association with intracellular molecules, including the ezrin-radixin-moesin actin-binding proteins, regulate functions mediated by PSGL-1 in leukocytes stimulated by chemoattractants.
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影响因子：
20.3
作者：
Laszik, Z;Jansen, PJ;Moore, KL
通讯作者：
Moore, KL
影响因子：
7.5
作者：
ROSEN, SD;BERTOZZI, CR
通讯作者：
BERTOZZI, CR
人中性粒细胞对 C5a 的反应：人 C5ades Arg-74 的寡糖部分在生物活性中起作用，但 C5a 的寡糖部分不起作用。
DOI：
--
发表时间：
1981
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Gerard,C;Chenoweth,DE;Hugli,TE
通讯作者：
Hugli,TE
影响因子：
3.3
作者：
Nakamura, F;Huang, LQ;Furthmayr, H
通讯作者：
Furthmayr, H
影响因子：
7.5
作者：
Bretscher, A
通讯作者：
Bretscher, A