Monocytes mediate Salmonella Typhimurium-induced tumor growth inhibition in a mouse melanoma model.
Monocytes mediate Salmonella Typhimurium-induced tumor growth inhibition in a mouse melanoma model.
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DOI：
10.1002/eji.202048913
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发表时间：
2021-12
影响因子：
5.4
通讯作者：
中科院分区：
文献类型：
--
作者：
近年来，细菌作为癌症替代疗法的使用已被重新研究。SL7207：具有免疫刺激潜力的营养缺陷型肠道沙门氏菌血清型鼠伤寒aroA突变体已被证明是用于此目的的有希望的菌株。在这里，我们表明全身施用SL 7207在体内诱导黑色素瘤肿瘤生长停滞，与对照PBS处理的小鼠相比，SL 7207处理组的存活率更高。SL 7207给药伴随着肿瘤浸润细胞的免疫表型向促炎方向的变化，TH 1细胞因子IFN-γ、TNF-α和IL-12的表达显著增加。有趣的是，Ly 6C +MHCII+单核细胞在SL 7207治疗后被募集到肿瘤中，并且是促炎性的。因此，使用氯膦酸盐脂质体消除这些浸润的单核细胞阻止了SL 7207诱导的肿瘤生长抑制。这些数据证明了浸润性炎性单核细胞在细菌介导的肿瘤生长抑制中的先前未被认识到的作用。这些信息突出了单核细胞在控制肿瘤生长中可能的新作用，有助于我们理解成功的癌症免疫治疗所需的免疫反应。 鼠伤寒沙门氏菌可以靶向肿瘤并在小鼠模型中阻止其生长，这一过程与炎症单核细胞的肿瘤浸润有关。消除吞噬细胞的氯膦酸盐脂质体抑制沙门氏菌介导的肿瘤生长抑制，表明这些细胞在沙门氏菌的抗肿瘤特性中发挥作用。
The use of bacteria as an alternative cancer therapy has been reinvestigated in recent years. SL7207: an auxotrophic Salmonella enterica serovar Typhimurium aroA mutant with immune‐stimulatory potential has proven a promising strain for this purpose. Here, we show that systemic administration of SL7207 induces melanoma tumor growth arrest in vivo, with greater survival of the SL7207‐treated group compared to control PBS‐treated mice. Administration of SL7207 is accompanied by a change in the immune phenotype of the tumor‐infiltrating cells toward pro‐inflammatory, with expression of the TH1 cytokines IFN‐γ, TNF‐α, and IL‐12 significantly increased. Interestingly, Ly6C+MHCII+ monocytes were recruited to the tumors following SL7207 treatment and were pro‐inflammatory. Accordingly, the abrogation of these infiltrating monocytes using clodronate liposomes prevented SL7207‐induced tumor growth inhibition. These data demonstrate a previously unappreciated role for infiltrating inflammatory monocytes underlying bacterial‐mediated tumor growth inhibition. This information highlights a possible novel role for monocytes in controlling tumor growth, contributing to our understanding of the immune responses required for successful immunotherapy of cancer. Salmonella Typhimurium can target tumors and stall their growth in mouse models, a process associated with tumor infiltration by inflammatory monocytes. Clodronate liposomes, which abrogate phagocytic cells, inhibit Salmonella‐mediated tumor growth inhibition, suggesting a role for these cells in Salmonella’s antitumor properties.
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DOI：
10.1128/genomea.01591-16
发表时间：
2017-02-02
期刊：
Genome announcements
影响因子：
--
作者：
Johnson SA;Ormsby MJ;Wall DM
通讯作者：
Wall DM
DOI：
10.1073/pnas.0408197102
发表时间：
2005-01-11
影响因子：
11.1
作者：
Chen, ML;Pittet, MJ;Khazaie, K
通讯作者：
Khazaie, K
肿瘤相关巨噬细胞的细胞和分子起源。
DOI：
10.1126/science.1252510
发表时间：
2014-05-23
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Franklin RA;Liao W;Sarkar A;Kim MV;Bivona MR;Liu K;Pamer EG;Li MO
通讯作者：
Li MO
影响因子：
11.2
作者：
Coffelt, Seth B.;Tal, Andrea O.;Lewis, Claire E.
通讯作者：
Lewis, Claire E.
影响因子：
4.7
作者：
Belai, Eduardo Bertoli;de Oliveira, Carine Ervolino;Campanelli, Ana Paula
通讯作者：
Campanelli, Ana Paula