Synthetic oligodeoxynucleotide probes for the rapid detection of bacteria associated with human periodontitis.
Synthetic oligodeoxynucleotide probes for the rapid detection of bacteria associated with human periodontitis.
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用于快速检测与人类牙周炎相关的细菌的合成寡脱氧核苷酸探针。
DOI：
10.1099/00221287-134-7-1931
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
U. Göbel
中科院分区：
文献类型：
--
作者：
P. Chuba;K. Pelz;G. Krekeler;T. S. Isele;U. Göbel
最近对牙龈下微生物群的分析表明放线菌（血友菌）放线菌和几种黑色拟杆菌与青少年、成人和快速进展的牙周炎的病因学有关。这些细菌的生长缓慢和挑剔的性质阻碍了快速细菌学诊断。为了构建诊断探针，对a (h)放线菌comitans、嗜aphrophilus、牙龈拟杆菌（Bacteroides gingivalis）、中间拟杆菌（Bacteroides intermedius）亚群II、溶糖拟杆菌（Bacteroides asaccharolyticus）和几个近缘种的16S rRNA分子进行了双脱氧测序。接下来，合成与16S rRNA中具有相当进化差异的特定区域互补的寡脱氧核苷酸，用作分子探针。在斑点杂交试验中，每个构建探针的物种的所有菌株都被正确识别，检测限小于5 x 10(3)个生物体。未观察到与近缘种（嗜蚜嗜血杆菌和嗜嗜嗜血杆菌除外）或污染菌的交叉杂交。使用改进的DNA/RNA杂交技术，可以在不到12小时内进行检测，而使用传统的细菌学方法则需要2-3周。
Analysis of the subgingival microflora has recently implicated Actinobacillus (Haemophilus) actinomycetemcomitans and several black Bacteroides species in the aetiology of juvenile, adult and rapidly progressing periodontitis. Rapid bacteriological diagnosis has been hampered by the slow growth and fastidious nature of these bacteria. To construct diagnostic probes, dideoxy sequencing of the 16S rRNA molecules from A. (H.) actinomycetemcomitans, Haemophilus aphrophilus, Bacteroides gingivalis, Bacteroides intermedius subgroup II, Bacteroides asaccharolyticus and several closely related species was performed. Next, oligodeoxynucleotides, complementary to defined regions of the 16S rRNA exhibiting considerable evolutionary divergence, were synthesized for use as molecular probes. In a dot-blot hybridization assay, all strains from each of the species for which probes were constructed were correctly identified, with a detection limit of less than 5 x 10(3) organisms. No cross-hybridization to closely related species (except for H. aphrophilus and Haemophilus paraphrophilus) or contaminating bacteria was observed. Using a modified DNA/RNA hybridization technique, the detection could be performed in less than 12 h, as compared to 2-3 weeks using conventional bacteriological procedures.
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成人牙周炎治疗后某些龈下细菌与牙周袋深度和牙周附着获得或丧失的关系。
影响因子：
6.7
作者：
Slots,J;Emrich,LJ;Genco,RJ;Rosling,BG
通讯作者：
Rosling,BG
DOI：
10.1073/pnas.82.20.6955
发表时间：
1985-01-01
影响因子：
11.1
作者：
LANE, DJ;PACE, B;PACE, NR
通讯作者：
PACE, NR
应用分子遗传学：微生物学家和临床医生的新工具。
DOI：
10.1093/infdis/153.3.416
发表时间：
1986
期刊：
The Journal of infectious diseases
影响因子：
--
作者：
Eisenstein,BI;Engleberg,NC
通讯作者：
Engleberg,NC
用于鉴定拟杆菌属物种的染色体 DNA 探针。
DOI：
10.1099/00221287-133-6-1423
发表时间：
1987
期刊：
Journal of general microbiology
影响因子：
--
作者：
Roberts,MC;Moncla,B;Kenny,GE
通讯作者：
Kenny,GE
影响因子：
4.3
作者：
CHRISTERSSON, LA;SLOTS, J;GENCO, RJ
通讯作者：
GENCO, RJ